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目的了解小双节病毒在成都市腹泻婴幼儿中的感染情况。方法收集成都市儿童医院、成都市第三人民医院2010年7-12月腹泻婴幼儿(≤5岁)粪便样本428份,用实时荧光RT-PCR方法检测小双节病毒,同时检测轮状病毒、诺如病毒、札如病毒、人星状病毒和肠道腺病毒。结果检出小双节病毒感染36份,检出率为8.41%(36/428)。其中Ⅰ型9份、Ⅱ型25份、Ⅰ和Ⅱ型共同感染2份,检出率分别为2.10%(9/428)、5.84%(25/428)和0.47%(2/428)。小双节病毒与其他腹泻病毒合并感染共31份。调查期间小双节病毒检出高峰为8、9月,主要感染2岁以下婴幼儿。结论小双节病毒是引起成都市婴幼儿腹泻的病原之一,以小双节病毒Ⅱ型为主,2岁以下婴幼儿是其高发人群,8、9月为其下半年高发季节。
Objective To understand the infection of small double-dengue virus in infants and young children in diarrhea in Chengdu. Methods A total of 428 stool samples from infants and young children (≤5 years) with diarrhea in Chengdu Children’s Hospital and Third People’s Hospital of Chengdu from 2010 to 2010 were collected. Real-time fluorescent RT-PCR method was used to detect the small- , Norovirus, Sapporo virus, human astrovirus and enteric adenovirus. Results 36 cases of small double-stranded virus infection were detected, the detection rate was 8.41% (36/428). Among them, 9 were type Ⅰ, 25 were type Ⅱ and 2 were co-infected with type Ⅰ and type Ⅱ, the detection rates were 2.10% (9/428), 5.84% (25/428) and 0.47% (2/428) respectively. Small joint viruses and other diarrhea virus infection with a total of 31 copies. Small double detection during the investigation of the virus peaked in August and September, the main infection of infants under 2 years of age. Conclusions The small double-stranded virus is one of the causative agents of infantile diarrhea in Chengdu. It is predominated by small bivalve virus type Ⅱ and infants under 2 years of age are the high incidence population. In August and September, it is the high incidence season in the second half of the year.