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目的 观察白介素－１（ＩＬ－ １）及地塞米松对人下颌骨髁状突软骨（ＭＣＣ） 细胞增殖、代谢及前胶原基因表达的影响。 方法 采用体外细胞培养、同位素掺入及狭缝杂交方法。 结果 ＩＬ－１ 具有抑制细胞增殖及胶原蛋白合成的作用，地塞米松一方面可促进细胞增殖，但同时却抑制了其胶原合成。二者在对前胶原基因表达影响方面作用相似，均可显著抑制软骨特征性的Ⅱ型胶原基因表达（分别为对照组的４８．６ ％ 和５７．９ ％） ，而提高非软骨的Ⅰ、Ⅲ型胶原基因表达。 结论 ＩＬ－ １ 对ＭＣＣ细胞分化、增殖及胶原合成的抑制作用可能是它在骨关节病发病中重要的致病途径之一。地塞米松具有一定促进细胞增殖的作用，但鉴于其对软骨细胞胶原合成及Ⅱ型胶原基因表达的抑制，提示临床上应用不当可能会诱发或加重骨关节病及软骨损害。
Objective To observe the effects of interleukin-1 (IL-1) and dexamethasone on proliferation, metabolism and procollagen gene expression in human mandibular condylar cartilage (MCC). Methods In vitro cell culture, isotope incorporation and slot hybridization methods. Results IL-1 inhibited cell proliferation and collagen synthesis. Dexamethasone, on the one hand, promoted cell proliferation but at the same time inhibited collagen synthesis. Both of them had similar effects on procollagen gene expression, both of which could significantly inhibit the expression of type Ⅱ collagen gene (48.6% and 57.9%, respectively) of cartilage, while the increase of non-cartilage Ⅰ, Type Ⅲ collagen gene expression. Conclusion The inhibitory effect of IL-1 on the differentiation, proliferation and collagen synthesis of MCC cells may be one of the important pathogenic mechanisms in the pathogenesis of osteoarthrosis. Dexamethasone has a certain role in promoting cell proliferation, but given its inhibition of chondrocyte collagen synthesis and type II collagen gene expression, suggesting improper clinical application may induce or aggravate osteoarthritis and cartilage damage.