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目的 :探讨EB病毒 (EBV)感染对鼻咽癌细胞系生长和细胞凋亡的影响。方法 :用EBV直接感染人鼻咽癌细胞系CNE1;用免疫组化 (LSAB)法检测EBV -LMP1和bcl- 2蛋白的表达 ;用MTT法测定鼻咽癌细胞系的生长能力 ;用流式细胞术和TUNEL法检测癌细胞凋亡。结果 :感染EBV的鼻咽癌细胞系 (E -CNE1)的EBV -LMP1表达阳性 ,生长能力较未感染EBV的CNE1明显增强 (P <0 0 1) ,2种鼻咽癌细胞系均无凋亡发生 ,而均仅有 2 %～ 3%的细胞表达bcl- 2蛋白。结论 :EBV感染和LMP1表达可促进鼻咽癌细胞生长 ,但对鼻咽癌细胞的bcl- 2表达和细胞凋亡无影响。
Objective: To investigate the effects of Epstein-Barr virus (EBV) infection on the growth and apoptosis of nasopharyngeal carcinoma cell lines. Methods: EBV was used to directly infect human nasopharyngeal carcinoma cell line CNE1. The expression of EBV-LMP1 and bcl-2 protein was detected by immunohistochemistry (LSAB) method. The growth of nasopharyngeal carcinoma cell line was determined by MTT assay. Cytometry and TUNEL assay of cancer cell apoptosis. Results: The EBV-LMP1 expression in EBV-infected nasopharyngeal carcinoma cell line (E-CNE1) was significantly higher than that in EBV-infected CNE1 (P <0.01) However, only 2% to 3% of cells expressed bcl-2 protein. CONCLUSION: EBV infection and LMP1 expression can promote the growth of nasopharyngeal carcinoma cells, but have no effect on the expression of bcl-2 and apoptosis of NPC cells.