论文部分内容阅读
采用RT-PCR方法克隆流行性造血器官坏死病病毒CH-1株MCP蛋白基因编码区,进而将膜外区编码基因片段,克隆于pET-32a,重组质粒pET-32a-MCP转化ROSETTA(DE3),经1.0 mmol/L IPTG诱导,外源基因获得高效表达。通过Western-blot试验证明表达产物具有良好的反应原性。
The coding region of MCP gene of epidemic hematopoietic necrosis virus CH-1 strain was cloned by RT-PCR. The gene encoding the outer membrane was cloned into pET-32a. The recombinant plasmid pET-32a-MCP was transformed into ROSETTA (DE3) After induced by 1.0 mmol / L IPTG, the foreign gene was highly expressed. Western-blot test proved that the expression product has good reactionogenicity.