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目的:评价内源性β-雌二醇在七氟烷麻醉致新生大鼠远期行为学异常中的作用。方法:清洁级健康雄性新生SD大鼠45只，5日龄，体重10～15 g，采用随机数字表法分为3组(n n＝15)：对照组(C组)正常笼内喂养，不接受麻醉；七氟烷组(S组)2.1%七氟烷麻醉6 h；福美司坦组(F组)麻醉前30 min皮下注射β-雌二醇合成抑制剂福美司坦2 mg/kg。C组和S组于麻醉前30 min皮下注射等量生理盐水。于出生后60 d时行高架十字迷宫(EPM)实验，出生后70 d时行前脉冲抑制(PPI)实验。n 结果:与C组比较，S组EPM实验开放臂停留时间百分比降低，PPI实验前脉冲比降低(n P<0.05)；与S组比较，F组EPM实验开放臂停留时间百分比升高，PPI实验前脉冲比增加(n P<0.05)。n 结论:内源性β-雌二醇参与了七氟烷麻醉致新生大鼠远期行为学异常的过程。“,”Objective:To evaluate the role of endogenous β-estradiol in long-term behavioral abnormalities induced by sevoflurane anesthesia in neonatal rats.Methods:Forty-five clean-grade healthy male Sprague-Dawley rats, aged 5 days, weighing 10-15 g, were divided into 3 groups (n n=15 each) using a random number table method: control group (group C), sevoflurane group (group S), and formestane group (group F). The rats were commonly reared in the cage in group C. Animals were exposed to 2.1% sevoflurane for 6 h in group S. In group F, formestane (estradiol synthesis inhibitor) 2 mg/kg was subcutaneously injected at 30 min before anesthesia.The equal volume of normal saline was subcutaneously injected at 30 min before anesthesia in C and S groups.The elevated plus maze (EPM) test was performed at 60 days after birth, and prepulse inhibition (PPI) test at 70 days after birth.n Results:Compared with group C, the percentage of time of staying at the open arm was significantly decreased, and the pulse rate before PPI test was decreased in group S (n P<0.05). Compared with group S, the percentage of time of staying at the open arm was significantly increased, and the pulse rate before PPI test was increased in group F (n P<0.05).n Conclusion:Endogenous β-estradiol is involved in the process of long-term behavioral abnormalities induced by sevoflurane anesthesia in neonatal rats.