论文部分内容阅读
以早抽薹品种CT07和晚抽薹品种T0601为亲本构建F2分离群体,采用分离集团混合分析法(Bulkedsegregant analysis,BSA)筛选与菜薹抽薹基因连锁的分子标记。用45条ISSR(Inter-simple sequence repeat,简单重复序列间扩增)引物和20对SRAP(Sequence related amplified polymorphism,序列相关扩增多态性)引物组合进行PCR扩增筛选,其中UBC834、UBC876、E13M4和E5M7对亲本和DNA池的扩增图谱表明,4个引物的差异条带均出现在早抽薹品种CT07和早现蕾(抽薹)池中。经F2代群体单株验证,4个标记均与早抽薹基因相连锁,且标记E13M4最近,距离为16.8 cM,这为菜薹抽薹基因的定位和分子标记辅助育种奠定了基础。
F2 segregation population was constructed with early bolting CT07 and late bolting cultivar T0601, and the molecular markers linked to the bolting gene of flowering cabbage were screened by Bulked segregant analysis (BSA). A total of 45 ISSR (Inter-simple sequence repeat) primers and 20 pairs of SRAP (Sequence related amplified polymorphism) primer combinations were selected for PCR amplification. UBC834, UBC876, The amplified bands of E13M4 and E5M7 on the parental and DNA pools showed that the four bands of the four primers appeared in the early bolting cultivar CT07 and the early budding (bolting) pool. After F2 population test, all four markers were linked to the early bolting gene, and the marker E13M4 was recently located at a distance of 16.8 cM, which lays a foundation for the bolting boll gene and molecular marker-assisted breeding.