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目的探讨微小卫星ＤＮＡ不稳定性（ＭＩＮ）与胃癌术前分期的关系。方法应用超声内镜对７３例胃癌患者行术前分期，同时采用银染ＰＣＲＳＳＣＰ方法检测内镜活检组织第２、５、１７号染色体４个位点的ＭＩＮ。结果ＭＩＮ总的检出率为３７０％，其中出现４、３、２个及单个位点异常分别为１１１％、２２２％、４８１％和１８５％。Ｔ１至Ｔ４期癌ＭＩＮ检出率分别为４０４％、３３３％、３９３％和３５７％（Ｐ＞００５），与胃癌的浸润深度无关，其中有淋巴结转移组为５１４％，明显高于无转移组的２２２％（Ｐ＜００２５）。结论ＭＩＮ是胃癌病变中常见的分子遗传学事件，与胃癌转移有一定的相关性。
Objective To investigate the relationship between microsatellite DNA instability (MIN) and preoperative staging of gastric cancer. Methods Preoperative staging was performed on 73 cases of gastric cancer patients by endoscopic ultrasonography. At the same time, the PCR-SSCP method with silver staining was used to detect the MIN of the 4 sites on chromosomes 2, 5, and 17 of the endoscopic biopsy. Results The total detection rate of MIN was 37.0%, of which 4, 3, 2 and single site abnormalities were 11.1%, 22.2%, 48.1%, and 18.5%, respectively. The detection rates of MIN from T1 to T4 were 40.4%, 33.0%, 39.5%, and 35.7%, respectively (P>0. 05), which were not associated with the depth of invasion of gastric cancer. Among them, there were lymph node metastases. It was 51.4%, which was significantly higher than 22% of the non-metastatic group (P<0. 025). Conclusion MIN is a common molecular genetic event in gastric cancer and has a certain correlation with gastric cancer metastasis.