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目的通过豚鼠实验模型研究上气道变应性炎症对下气道的影响。方法12只豚鼠分为2组,每组6只,鼻炎组豚鼠给予鼻腔卵蛋白/氢氧化铝滴注(连续10 d,每天2次)。间隔1周后,鼻腔给予2%卵蛋白每日滴注,连续7 d;对照组均使用生理盐水替代卵蛋白。最后一次激发后10 min,对喷嚏、抓鼻、流涕等症状量化评估,观察肺部症状;抽取动物血清,ELISA方法测定IgE和IL-5水平;计量鼻腔黏膜及肺部黏膜嗜酸粒细胞浸润数量,观察鼻腔和肺组织的病理改变。结果鼻炎组豚鼠明显出现喷嚏、鼻痒、挠鼻、流涕等典型症状,但肺部哮喘症状不明显;血清IgE、IL-5水平升高;鼻腔和肺部黏膜见多量嗜酸粒细胞浸润,且出现病理结构变化。对照组未出现上述变化。结论上气道变应性炎症可导致下气道发生类似的炎症反应,并导致免疫学改变。
Objective To study the effects of upper airway allergic inflammation on the lower airway by guinea pig experimental model. Methods Twelve guinea pigs were divided into 2 groups, 6 in each group. The rhinitis group received intranasal egg albumin / aluminum hydroxide infusion (twice daily for 10 days). One week later, 2% ovalbumin was given to the nasal cavity daily for 7 days. In the control group, normal saline was used instead of ovalbumin. 10 min after the last challenge, quantitative evaluation of symptoms such as sneezing, nasal salvage and runny nose was performed to observe the symptoms of the lungs. Serum of the animals was taken and the levels of IgE and IL-5 were measured by ELISA. Nasal mucosa and pulmonary mucosal eosinophils Infiltration number, observe the pathological changes of the nasal cavity and lung tissue. Results In the rhinitis group, typical symptoms such as sneezing, nasal itching, scratching nose and runny nose were obvious, but the symptom of pulmonary asthma was not obvious. The level of IgE and IL-5 in serum increased. The infiltration of nasal mucosa and pulmonary mucosa , And pathological changes occurred. The control group did not appear the above changes. Conclusion Upper airway allergic inflammation can cause a similar inflammatory response in the lower airway and lead to immunological changes.