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目的研究氯化镉对雄性小鼠生殖器官和生殖细胞的毒性作用以及对雄性小鼠生殖功能的影响。方法分别以每公斤体重0..5mg/kg、2mg/kg、8mg/kg体重腹腔染毒4周龄雄性小鼠,共10次。第50天以雌雄2:1同笼交配,观察雌鼠受孕率、生育子胎数、胎鼠重量。同时观察染毒雄鼠第50天时睾丸发育和睾丸指数、附睾精子数量、精子活动率和精子畸形率以及生殖细胞减数分裂。结果2mg/kg和8mg/kg组染毒小鼠睾丸发育受到影响,睾丸指数显著低于对照组和0.5mg/kg组(p<0.05,p<0.001)。2mg/kg组中部分小鼠(6/11)和8mg/kg组存活小鼠睾丸发育不良,无精子,不育。2mg/kg组小鼠1周受孕率和3周受孕率显著低于对照组和0.5mg/kg组(p<0.05),但染毒组异常妊娠率与对照组比差异无显著性。0.5mg/kg组雄鼠生育力、附睾精子数量、精子活动率和精子畸形率与对照组比较差异无显著性。结论2mg/kg浓度和更高浓度氯化镉致4周龄雄鼠睾丸发育不良,导致无精子症和不育,是造成生殖力降低的主要原因。
Objective To study the toxic effects of cadmium chloride on reproductive organs and germ cells of male mice and the effects on reproductive function of male mice. Methods Male 4-week-old mice were peritoneally injected with body weight of 0..5mg / kg, 2mg / kg and 8mg / kg respectively for 10 times. The first 50 days of male and female 2: 1 mating with the cage, observed female fertility rate, the number of childbirth child, fetal rat weight. At the same time, testicular development and testicular index, epididymal sperm count, sperm motility and sperm deformity rate, and germ cell meiosis were observed at the 50th day in exposed male rats. Results The development of testis in 2 mg / kg and 8 mg / kg groups was affected, and the testicular index was significantly lower than that in control group and 0.5 mg / kg group (p <0.05, p <0.001). Some mice in the 2 mg / kg group (6/11) and the 8 mg / kg group showed hypoplastic testicles, no sperm, and infertility. The 1-week conception rate and 3-week conception rate in 2mg / kg group were significantly lower than those in control group and 0.5mg / kg group (p <0.05). However, there was no significant difference in the abnormal pregnancy rate between the 2mg / kg group and the control group. The fertility, epididymal sperm count, sperm motility rate and sperm deformity in male rats at 0.5 mg / kg group were not significantly different from those in control group. Conclusions 2 mg / kg cadmium chloride and 4-week-old male caduceus lead to azoospermia and infertility in male testes, which is the main reason for the decrease of reproductive performance.