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目的：观察芹灵冲剂（ＱＬ） 在鸭体内对鸭乙型肝炎病毒（ＤＨＢＶ） － ＤＮＡ 的抑制效果。方法：用ＤＨＢＶ感染雏鸭进行药物治疗试验，芹灵冲剂设３ 个剂量组，疗程１０ｄ，分离血清，样品点膜，标记ＤＨＢＶ－ ＤＮＡ探针，（ 血清） 斑点杂交，放射自显影膜片斑点检测。以杂交斑点光密度值（ＯＤ 值） 进行自身和组间比较，并作鸭肝病理检查评价。结果：芹灵冲剂高剂量组（５、１０ｇ·ｋｇ－１）能降低ＤＨＢＶ感染鸭血清ＤＨＢＶ－ ＤＮＡ 水平，给药ｄ５ 和ｄ１０ 的抑制率与病毒对照组比较，均有极显著性差异（ Ｐ ＜０ ．００１） ，并呈量效关系。低剂量组（２．５ｇ·ｋｇ－１）也有一定的疗效。肝病理镜检结果证实，空白对照组鸭肝细胞的变性坏死较重，而芹灵冲剂治疗组（ 大、中剂量） 则较轻。结论：芹灵冲剂不仅对ＤＨＢＶ有明显的抑制作用，而且有保护鸭肝细胞的作用。
Objective: To observe the inhibitory effect of QL on duck hepatitis B virus (DHBV)-DNA. METHODS: DHBV was used to infect ducklings for drug treatment. Three dosage groups were used for the preparation of Qinling granules. The duration of treatment was 10 days. Serum was separated, sample spots were spotted, labeled with DHBV-DNA probes, (blood) dot blot hybridization, and autoradiographic patch spots. Testing. The optical density (OD value) of the hybrid spots was used to compare the differences between the groups and the groups, and the duck liver pathology was evaluated. Results: The high-dose group (5,10 g·kg-1) of Qinling Granules could reduce the serum DHBV-DNA level in DHBV-infected ducks. The inhibition rates of d5 and d10 administration were significantly different from those in the virus control group (P <0.001) and showed a dose-effect relationship. Low dose group (2.5g·kg-1) also has a certain effect. Liver pathological examination confirmed that the denatured and necrotic hepatocytes of the ducks in the blank control group were heavier, while those in the QLH treatment group (large and medium doses) were lighter. Conclusion: Qinling granule not only has obvious inhibitory effect on DHBV, but also protects duck liver cells.