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目的研究丙烯酰胺(AA)急性染毒对雄性小鼠生殖细胞微管蛋白的影响。方法 36只健康雄性小鼠,随机分为阴性对照组,15、25和50 mg/kg剂量组,每组9只,腹腔注射连续染毒6 d,间接免疫荧光技术测睾丸细胞微管蛋白的表达量。结果不同剂量AA染毒后小鼠体重增加,睾丸与体重的比值差异无统计学意义(F=0.878,P=0.466)。生殖细胞微管蛋白与对照组相比,15 mg/kg组差异无统计学意义(P>0.05),25 mg/kg组和50 mg/kg组差异具有统计学意义(P<0.05)。结论在本实验条件下,AA染毒对小鼠生殖细胞微管蛋白产生毒性影响,且存在剂量效应关系。
Objective To investigate the effects of acute exposure to acrylamide on germ cell tubulin in male mice. Methods Thirty-six healthy male mice were randomly divided into the negative control group, 15, 25 and 50 mg / kg dose groups, 9 in each group. The mice were injected intraperitoneally consecutively for 6 days. Indirect immunofluorescence was used to detect the expression of tubulin The amount of expression. Results After the mice were exposed to different doses of AA, their body weight increased and the ratio of testis to body weight had no significant difference (F = 0.878, P = 0.466). There was no significant difference in germ cell tubulin between the control group and the 15 mg / kg group (P> 0.05). The differences between the 25 mg / kg group and the 50 mg / kg group were statistically significant (P <0.05). Conclusion Under the experimental conditions, AA toxicity on mouse germ cell tubulin toxicity, and there is a dose-response relationship.