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目的 探讨肠道病毒感染在多发性肌炎 /皮肌炎 (PM/DM)发病机理中的作用和意义。方法 用逆转录聚合酶链反应 (RT PCR)和中和试验分别检测 16例 PM/DM患者和 10例正常人对照组外周血中肠道病毒核糖核酸 (RNA)和抗体滴度。结果 16例 PM/DM患者中肠道病毒核糖核酸检出率为 50.0％，病毒中和试验中和抗体阳性率为 12.5％。正常人对照组中，仅 1例测到肠道病毒 RNA，中和试验均阴性。在有肌肉酸痛症状的患者 (11例 )中，肠道病毒 RNA的阳性率为 72.73％，明显高于中和抗体的阳性率 (18.18％ )；肠道病毒 RNA阳性组肌酸磷酸激酶 (CPK)升高率显著高于阴性组 (P0.05)；中和抗体阳性组与阴性组上述指标相比，差异均无显著性 (P >0.05)。结论 肠道病毒核糖核酸在 PM/DM患者中检出率高；并且在肌肉酸痛和 CPK升高的患者中，其阳性率也高，两者之间有一定内在联系。
Objective To investigate the role and significance of enterovirus infection in the pathogenesis of polymyositis / dermatomyositis (PM / DM). Methods RT-PCR and neutralization assays were used to detect the RNA and antibody titers in peripheral blood of 16 PM / DM patients and 10 normal controls respectively. Results The detection rate of enterovirus ribonucleic acid in PM / DM patients was 50.0% in 16 cases, and 12.5% in virus neutralization test. Normal control group, only 1 case of enterovirus RNA was detected, neutralization test were negative. In patients with muscle soreness (11 cases), the positive rate of enterovirus RNA was 72.73%, which was significantly higher than that of neutralizing antibody (18.18%); the positive rate of enterovirus RNA-positive group of creatine phosphokinase (CPK ) Was significantly higher than that in the negative group (P <0.05). There was no significant difference between the positive and negative groups (P> 0.05). Conclusion The detection rate of enterovirus RNA in PM / DM patients is high, and in patients with muscle ache and CPK, the positive rate is also high, and there is a certain relationship between the two.