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以高山红景天为试材,采用IMAGING-PAM调制叶绿素荧光成像系统,测定了转UDP-葡萄糖:类黄酮3-O-糖基转移酶基因FaGT1的高山红景天及转FaGT1的RNAi作用基因FaGT1i的高山红景天的叶绿素荧光参数,分析转UDP-葡萄糖:类黄酮3-O-糖基转移酶基因的高山红景天叶片的叶绿素荧光特性,为研究红景天种质改良提供科学依据。结果表明:当光照强度为55μmol·m~(-2)·s~(-1)时,转FaGT1基因高山红景天的光系统Ⅱ的最大量子产量与高山红景天对照组和转FaGT1i基因高山红景天无显著差异,光系统Ⅱ潜在活性、非光化学淬灭系数/光化学淬灭系数和实际量子产量显著高于高山红景天对照组和转FaGT1i的高山红景天,但是调节性能量耗散的量子产量、非调节性能量耗散的量子产量则相对较低。另外,通过在不同的光照强度下,对光系统Ⅱ电子传递速率、光化学淬灭系数和非光化学淬灭系数进行了分析,得出转FaGT1基因高山红景天的光系统Ⅱ电子传递速率、非光化学淬灭系数呈先上升后稳定的趋势,显著高于高山红景天对照组和转FaGT1i的高山红景天;但是光化学淬灭系数呈先下降后平稳的变化趋势,与对照及转FaGT1i基因高山红景天无显著差异。转FaGT1基因高山红景天的光系统Ⅱ原初光能转换效率和潜在的光合活力均增强,植物自身的能量代谢得到提高,该研究结果为高山红景天的新种质培育提供了科学依据。
Rhodiola sachalinensis was used as experimental material, and fluorescence quantitative PCR system of IMAGING-PAM was used to detect RNAi gene of Rhodiola sachalinensis and FaGT1 that transferred UDP-glucose: flavonoid 3-O-glycosyltransferase gene FaGT1 FaGT1i Rhodiola sachalinensis chlorophyll fluorescence parameters, the analysis of UDP-glucose: flavonoid 3-O-glycosyltransferase gene Alhsia Rhodiola leaves chlorophyll fluorescence characteristics, in order to provide a scientific basis for Rhodiola rosea germplasm improvement . The results showed that when the light intensity was 55μmol · m ~ (-2) s ~ (-1), the maximum quantum yield of photosystem Ⅱ of Rhodiola sachalinensis transformed with FaGT1 gene was significantly lower than that of the control group and FaGT1i gene Rhodiola sachalinensis had no significant difference. The potential activity of photoperiod Ⅱ, non-photochemical quenching coefficient / photochemical quenching coefficient and actual quantum yield were significantly higher than that of Rhodiola sachalinensis and Rhodiola sachalinensis transformed with FaGT1i, however, Dissipative quantum yield, non-regulatory energy dissipation quantum yield is relatively low. In addition, the electron transfer rate, photochemical quenching coefficient and non-photochemical quenching coefficient of Photosystem Ⅱ were analyzed under different light intensities, and the photosystem Ⅱ electron transfer rate of Rhodiola sachalinensis transferred to FaGT1 gene was obtained. The photochemical quenching coefficient showed the trend of first rising and then stabilizing, which was significantly higher than that of Rhodiola sachalinensis and Rhodiola sachalinensis transformed with FaGT1i. However, the photochemical quenching coefficient showed a steady trend of decrease first and then decreased with the increase of FaGT1i gene Rhodiola rosea no significant difference. The photosynthetic efficiency and potential photosynthetic activity of photosystem Ⅱ of Rhodiola sachalinensis transformed with FaGT1 gene were enhanced, and the energy metabolism of plants was enhanced. The results provided a scientific basis for the cultivation of new Rhodiola rosea germplasm.