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培养基和培养材料的消毒组织培养能否获得成功的第一个条件就是无菌操作。培养基实际上是最有利于细菌和真菌繁殖的地方,如果培养基落入了细菌,整个培养基都会受到污染。因此,在培养基配制好以后应瑟时灭菌。1.培养基的消毒一般培养基都采用高压锅来灭菌,在高压锅内温度达120℃,1.1公斤/厘米~2压力时,保持灭菌15—20分钟。也可以将培养基煮沸10分钟,24小时后再煮沸20分钟来进行灭菌。消过毒的培养基通常置于10℃下保存,特别是含有生长调节物质的培养基在低温(4—
Disinfection of culture media and culture material The first condition for successful tissue culture is aseptic manipulation. The medium is actually the most favorable place for bacteria and fungi to grow, and if the medium falls into bacteria, the entire medium is contaminated. Therefore, the medium should be sterilized after the preparation of the medium. 1. Culture medium disinfection are generally used autoclave to sterilize, in the pressure cooker temperature of 120 ℃, 1.1 kg / cm ~ 2 pressure, maintain sterilization for 15-20 minutes. It is also possible to sterilize the medium by boiling for 10 minutes, boiling for another 24 hours and then for 20 minutes. Sterile medium is usually stored at 10 ° C, especially medium containing growth regulators at low temperature (4-