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目的分析浙江省2004—2011年117名男男性接触者(MSM)HIV-1感染病毒的序列特征,了解HIV-1毒株类型和特征。方法从实验室留样的MSM HIV感染者血液中提取DNA或RNA,用巢式PCR或RT-PCR方法扩增gag、pol基因区片段,测定序列并分析。结果 117名MSM HIV感染者中,序列结果按户籍来源覆盖21个省(市、自治区),感染毒株包括CRF01_AE 99名(84.62%)、B亚型7名(5.98%)、CRF07_BC 6名(5.13%)、CRF08_BC和CRF59_01B各1名(0.85%)。3名(2.56%)疑似01_B重组毒株与安徽感染者在系统进化树上聚集成可靠的次级进化簇(99%),且具有类似重组断点模式。84个CRF01_AE毒株的pol区基因序列聚集成簇,形成多个可靠的次级进化簇(30个节点的bootstrap值高于70%)。结论浙江省MSM HIV感染者主要流行CRF01_AE毒株,该人群HIV感染率高,应加强监测。CRF59_01B和新的01B重组毒株在浙江首次检出。
Objective To analyze the sequence characteristics of HIV-1 infection in 117 MSM patients from 2004 to 2011 in Zhejiang Province, and to understand the type and characteristics of HIV-1 strains. Methods DNA or RNA was extracted from the blood of MSM-infected HIV-infected labs. The gag and pol gene regions were amplified by nested PCR or RT-PCR. The sequences were determined and analyzed. Results Among the 117 MSM HIV-infected patients, the sequences included 21 provinces (municipalities and autonomous regions) according to household registration. The infected strains included 99 (84.62%) of CRF01_AE, 7 (5.98%) of B subtypes, 6 of CRF07_BC 5.13%), one for CRF08_BC and one for CRF59_01B (0.85%). Three (2.56%) suspected 01_B recombinant strains and Anhui infected individuals clustered in the phylogenetic tree as a reliable secondary evolutionary cluster (99%) with similar recombination breakpoint patterns. The pol gene sequences of 84 CRF01_AE strains clustered into several reliable secondary evolutionary clusters (30 bootstrap values higher than 70%). Conclusion The predominant strains of CRF01_AE in HIV-infected MSM in Zhejiang Province are HIV-positive and should be monitored. CRF59_01B and the new 01B recombinant strains were detected in Zhejiang for the first time.