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目的评价卵泡刺激素和人绒毛膜促性腺激素在促排卵周期未成熟卵母细胞体外培养过程中的应用价值。方法收集控制性超促排卵周期行单精子显微注射(ICSI)过程中得到的未成熟卵母细胞(MⅠ期和GV期),随机分为加促性腺激素培养组和不加促性腺激素培养组,培养24~28 h后成熟卵母细胞进行ICSI授精,并观察胚胎发育情况,记录成熟率、受精率、卵裂率和优胚率。结果对于MⅠ期卵母细胞,是否使用促性腺激素不影响其24 h成熟率、受精率、分裂率和优胚率(P>0.05)。对于GV期卵母细胞,培养液中加入促性腺激素能提高优质胚胎率(31.3%vs 4.2%,P<0.05),但两组的成熟率、受精率和卵裂率差异无统计学意义(P>0.05)。结论控制性超促排卵中获得的未成熟卵母细胞进一步体外培养,无论是否添加促性腺激素都可自然成熟,在GV期卵母细胞的体外培养过程加入促性腺激素可获得更高的优胚率。
Objective To evaluate the value of follicle-stimulating hormone and human chorionic gonadotropin in the process of in vitro culture of immature oocytes during ovulation induction. Methods Immature oocytes (stage MⅠ and stage GV) obtained during ICSI were collected during the controlled ovarian hyperstimulation (OVX) cycle and were randomly divided into gonadotrophin group and gonadotrophin group Group, cultured 24 ~ 28 h after maturation of oocytes ICSI insemination, and observe the embryonic development, recording maturation rate, fertilization rate, cleavage rate and excellent embryo rate. Results For M Ⅰ oocytes, whether gonadotropin was used or not did not affect the 24 h ripening rate, fertilization rate, cleavage rate and excellent embryo rate (P> 0.05). For GV oocytes, the addition of gonadotrophin to the culture medium increased the rate of high quality embryos (31.3% vs 4.2%, P <0.05), but there was no significant difference in maturation rate, fertilization rate and cleavage rate between the two groups P> 0.05). Conclusions The immature oocytes obtained from controlled hyperstimulation are further cultured in vitro with or without gonadotrophin, and the addition of gonadotrophin to GV oocytes during in vitro culture can obtain higher excellent embryos rate.