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目的:探讨丙氨瑞林对子宫内膜癌裸鼠皮下移植瘤进行干预后对子宫内膜癌及移植瘤中TRB3基因表达的影响。方法:将体外培养的子宫内膜癌细胞株HEC-1-B接种在裸鼠的皮下,建立移植瘤模型,将32只雌性裸鼠随机分为4组,即低剂量组(15μg/kg)、中剂量组(30μg/kg)、高剂量组(60μg/kg)和对照组(生理盐水),各剂量组每日肌注1次,密切观察裸鼠及肿瘤的生长情况。给药6周后将裸鼠处死,取出移植瘤并测量其体积计算抑瘤率,采用免疫组化法检测瘤组织中TRB3基因的表达。结果:随着丙氨瑞林浓度的增大,抑瘤率逐渐增高,低、中、高剂量组的抑瘤率分别为(12.90±0.12)%、(25.05±0.08)%、(41.92±0.12)%,与对照组相比,差异均有统计学意义(P<0.05)。不同浓度的丙氨瑞林干预6周后,瘤组织中TRB3的表达有不同程度的下降,经两两比较,差异均有统计学意义(P<0.05)。结论:丙氨瑞林对裸鼠移植瘤的生长具有抑制作用,且随着丙氨瑞林剂量的增加,抑瘤作用逐渐增强。丙氨瑞林对肿瘤的抑制作用机制可能与下调移植瘤组织中TRB3基因的表达有关。
OBJECTIVE: To investigate the effect of ararelin on the expression of TRB3 in endometrial carcinoma and xenografts after intervention of subcutaneous xenografts of endometrial carcinoma in nude mice. Methods: HEC-1-B cells were inoculated subcutaneously in nude mice to establish a transplanted tumor model. 32 female nude mice were randomly divided into 4 groups, namely, low dose group (15μg / kg) , Middle dose group (30μg / kg), high dose group (60μg / kg) and control group (normal saline). Each dose group was intramuscularly injected once daily to observe the growth of nude mice and tumor. After 6 weeks, the nude mice were sacrificed, the xenografts were removed and their volume was measured to calculate the tumor inhibition rate. The expression of TRB3 gene was detected by immunohistochemistry. Results: With the increase of the concentration of the arerelin, the tumor inhibition rate gradually increased, and the inhibition rates of the low, medium and high dose groups were (12.90 ± 0.12)%, (25.05 ± 0.08)%, (41.92 ± 0.12 )%, Compared with the control group, the difference was statistically significant (P <0.05). The expression of TRB3 in the tumor tissue decreased with different concentrations of ararelin after 6 weeks, and the difference was statistically significant (P <0.05). CONCLUSION: Arirarelin can inhibit the growth of xenografted tumor in nude mice, and the anti-tumor effect gradually increases with the increase of the dose of ararelin. The mechanism of inhibition of angiotensin on angiogenesis may be related to down-regulation of TRB3 gene expression in xenograft tumors.