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目的探讨天然免疫与凝血相关分子的关系及其理论和其临床意义。方法采用细菌内毒素(LPS)高、低2个剂量分别注射C57BL/6正常小鼠和MyD88基因敲除小鼠,并对正常小鼠注射IL-6、IFN-γ细胞因子抗体,观察小鼠生存率,收获肝组织提取mRNA,RT-PCR的方式检测各相关细胞因子及凝血因子的表达;采用原位孵育实验检测PT(prothrombinase)/PA(plasminogen activator)/PC(protein C)活性;采用Western blot方法检测纤维蛋白沉积,将所有的实验结果使用医学统计软件进行作图分析。结果 IL-6、IFN-γ2种细胞因子抗体通过激活PA活性抑制过量纤维蛋白的生成,延长小鼠存活时间(P<0.003)。结论通过调节天然免疫相关因子的表达来调控凝血系统的功能,控制纤维蛋白适量生成,为治疗败血症提供数据支持。
Objective To investigate the relationship between innate immunity and coagulation-related molecules and its theory and clinical significance. Methods C57BL / 6 normal mice and MyD88 knockout mice were injected with high and low doses of bacterial endotoxin (LPS) respectively. Normal mice were injected with IL-6 and IFN-γ cytokine antibodies. Survival rate, mRNA extracted from liver tissue were harvested, and the expression of cytokines and coagulation factors were detected by RT-PCR. The activity of PT (prothrombinase) / PA (protein C) was detected by in situ incubation; Western blot was used to detect fibrin deposition, and all the results were plotted using medical statistics software. Results The IL-6 and IFN-γ cytokines could prolong the survival time of mice by activating PA activity and inhibiting the formation of excessive fibrin (P <0.003). Conclusion The regulation of the function of the coagulation system by regulating the expression of innate immune related factors and the control of the amount of fibrin production provide data support for the treatment of sepsis.