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目的观察小鼠肾集合管发育过程中主细胞的形态学变化及水通道蛋白(AQP)-2、-3的表达,探讨AQP-2、-3与小鼠肾集合管主细胞发育的关系及作用。方法应用透射电镜、免疫组织化学、免疫印迹技术并结合体视学方法,观察并检测胚龄16、18 d胎鼠及生后1、3、7、14、21 d仔鼠肾集合管发育过程中主细胞的形态学变化及AQP-2、-3的表达。结果小鼠胚龄18 d可见发育早期的主细胞。生后7～21d,主细胞形态结构发育基本完善。AQP-2于胚龄18 d表达于集合管主细胞游离面细胞膜和胞质内,生后1d其阳性表达逐渐增加,于生后21d达到高峰。AQP-3于胚龄16 d表达在主细胞的侧基底膜,生后1d达到较高水平。结论 AQP-2与AQP-3在肾发生发育中对水通透性的调节可能发挥了重要的作用,AQP-2的作用发生在出生后,AQP-3的作用主要发生在胚胎时期。
Objective To observe the morphological changes of primary cells and expression of aquaporin (AQP) -2 and -3 during the development of mouse collecting duct and to explore the relationship between the expression of AQP-2 and -3 and the development of mouse primary integrin cells. effect. Methods Transmission electron microscopy, immunohistochemistry and immunoblotting combined with stereological methods were used to observe and detect the development of renal collecting tube in embryos aged 16 and 18 days and postnatal days 1,3,7,14 and 21 In the main morphological changes and AQP-2, -3 expression. Results The mouse embryonic age 18 days visible early development of the main cells. From 7 to 21 days after birth, the morphological structure of the main cells developed basically. The expression of AQP-2 was detected on the free surface membrane and cytoplasm of integrin main tube cells 18 days after embryogenesis. The positive expression of AQP-2 at 1 day after birth gradually increased and reached a peak at 21 days after birth. AQP-3 expressed in the basal lamina of the main cell on the 16th day after embryo implantation and reached a high level on the 1st day after birth. Conclusions AQP-2 and AQP-3 may play an important role in the regulation of water permeability in renal development. The effect of AQP-2 occurs after birth, and the effect of AQP-3 mainly occurs in embryonic stage.