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目的:研究采用组织工程技术通过颌下腺细胞与明胶海绵复合物的体内移植,探讨在同种异体动物体内构建具有生命活力的组织工程化颌下腺的可行性。方法:选用Wistar大鼠18只,分三组。A组:明胶海绵材料与鼠颌下腺细胞复合培养物;B组:单纯明胶海绵材料;C组:空白切开缝合对照。材料植入大鼠背部,术后3、7和15 d分期观察植入物的大体形态、组织学和超微结构,BrdU标记表达,及免疫排斥反应变化。结果:A组植入体内后颌下腺细胞在材料生长,形成的组织表面白色且有光泽、致密,组织学可见材料排列较为规则,15 d细胞仍成活并大量增殖,BrdU表达为阳性;B组植入体内7 d后材料逐渐变细,15 d后材料被逐渐降解吸收出现中断;A组分别于3、7、15 d进行扫描电镜观察可见颌下腺细胞数目逐渐增多,黏附在材料表面上生长,伸出突起,并可见明显的细胞分裂增殖,细胞形成功能性团块;A、B两组与C组相比3、5、7 d时白细胞数量增多明显,但逐步降低,第15天三组白细胞数量趋于一致。三组淋巴细胞数量变化不大,无显著性差异。结论:同种异体颌下腺细胞与明胶海绵材料复合构建的组织工程颌下腺,植入免疫功能正常的动物体内,细胞具有生命力,能够在材料上黏附生长、增殖,免疫反应随时间增加逐渐趋于正常。
OBJECTIVE: To study the feasibility of constructing tissue-engineered submaxillary glands in allogeneic animals by means of tissue engineering through in vivo transplantation of submandibular gland cells and gelatin sponge complexes. Methods: 18 Wistar rats were selected and divided into three groups. Group A: gelatin sponge material and rat submandibular gland cell complex culture; Group B: simple gelatin sponge material; Group C: blank open suture control. Materials were implanted into the back of the rats. The gross morphology, histology and ultrastructure of the implants, BrdU labeling and the changes of immune rejection were observed at 3, 7 and 15 days after operation. Results: The cells in Group A were implanted into the body of the submandibular gland cells. The surface of the cells was white and shiny, and the histology showed that the arrangement of materials was more regular. After 15 days, the cells survived and proliferated, BrdU was positive. After 7 days, the material became finer and the material was gradually degraded and absorbed after 15 days. In group A, the number of submandibular gland cells gradually increased after 3 and 7 days and 15 days respectively. The number of leukocytes increased significantly at 3, 5 and 7 days in group A and group B, but gradually decreased. On the 15th day, the number of white blood cells The number tends to be the same. Three groups of lymphocytes changed little, no significant difference. CONCLUSION: The tissue-engineered submandibular gland constructed by allogeneic submandibular gland cells and gelatin sponge material is implanted into animals with normal immune function. The cells are viable and can adhere and grow on the materials. The immune response gradually becomes normal with time.