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目的建立同时测定仙茅中多种酚苷类化合物的HPLC方法。方法 Angilent 1100高效液相色谱仪,Zorbax SB C18色谱柱;检测波长:230 nm;柱温:30℃;进样量20μL;流速1 mL.min-1;流动相:乙腈(A)-0.1%醋酸水溶液(B),梯度洗脱程序为:0～25 min,4%～6%A;25～58 min,6%～17%A;58～85 min,17%～22%A。结果 8种酚苷成分5-羟甲基糠醛(1),2-羟基-5-(2-羟乙基)苯基-β-D-吡喃葡萄糖苷(2),苔黑酚龙胆二糖苷(3),苔黑酚葡萄糖苷(4),苔黑酚-1-O-β-D-呋喃芹糖基-(1→6)-β-D-吡喃葡萄糖苷(5),2,6-二甲氧基苯甲酸(6),仙茅苷(7)和仙茅素A(8)的质量浓度和峰面积呈良好的线性关系,平均加样回收率符合含量测定要求。结论该方法简便、灵敏、重复性好,适用于同时测定仙茅中多种酚苷类成分。
OBJECTIVE To establish a HPLC method for simultaneous determination of various phenolic compounds in Curculigo lactiflora. The method was carried out on a Zorbax SB C18 column with an Agilent 1100 high performance liquid chromatograph. The detection wavelength was 230 nm. The column temperature was 30 ℃. The injection volume was 20 μL. The flow rate was 1 mL.min-1. Acetic acid solution (B), gradient elution program: 0 ~ 25 min, 4% ~ 6% A; 25 ~ 58 min, 6% ~ 17% A; 58 ~ 85 min, 17% ~ 22% Results Eight kinds of phenolic compounds 5-hydroxymethylfurfural (1), 2-hydroxy-5- (2-hydroxyethyl) phenyl -β-D-glucopyranoside (2) Glycosides (3), orcinol glucoside (4), orcinol-1-O-β-D-furanosyl- (1 → 6) , 6-dimethoxybenzoic acid (6), curculin (7) and curculin A (8) showed a good linear relationship with the peak area, and the average recovery rate was in accordance with the determination of content. Conclusion The method is simple, sensitive and reproducible. It is suitable for the simultaneous determination of a variety of phenolic compounds in Curculigo orchids.