论文部分内容阅读
玉米雄性不育材料是一种宝贵的种质资源,不育基因的遗传分析与定位研究对玉米分子育种和杂种优势利用具有重要价值。通过对从美国引进的玉米雄性不育突变体材料ms14进行雄花育性鉴定和花药I_2-KI染色,表明该突变体是无花粉型雄性不育;通过不育突变体ms14与正常自交系郑58、昌7-2杂交获得F_1,然后自交构建两个F_2遗传分离群体(ms14×郑58和ms14×昌7-2),并进行雄花育性调查、数据统计和遗传分析,发现可育株数与不育株数的分离比是3∶1,表明该突变体由隐性单基因控制;通过SSR等分子标记与不育位点的连锁分析,将ms14基因定位在玉米第1染色体的SSR标记umc2025和umc1676之间,遗传距离分别是2.2c M和0.3c M。对玉米不育基因ms14的遗传分析和初步定位,为该基因的精细定位和克隆、不育机理的解析及其产业化应用奠定了基础。
Maize male sterile material is a valuable germplasm resource. Genetic analysis and mapping of sterile genes are of great value to maize molecular breeding and heterosis utilization. The maize male sterile mutant maize MS14 introduced from the United States was identified by anther fertility and anther I_2-KI staining. The results showed that the mutant was pollen-type male sterile. The male sterile MS14 was crossed with the normal inbred line Zheng 58 and Chang 7-2 to obtain F_1, and then self-constructed two F_2 genetic segregation populations (ms14 × Zheng 58 and ms14 × Chang 7-2), and conducted male fertility surveys, data statistics and genetic analysis, and found fertile The segregation ratio of the number of plants to the number of sterile plants was 3: 1, indicating that the mutant was controlled by a recessive single gene. By linkage analysis of SSR markers and sterile sites, the ms14 gene was mapped on the SSR marker of maize chromosome 1 Between umc2025 and umc1676, the genetic distances were 2.2c M and 0.3c M, respectively. The genetic analysis and preliminary mapping of the maize sterile gene ms14 laid the foundation for the fine mapping and cloning of the gene, the analysis of its sterility mechanism and its industrial application.