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24只BALB/c小鼠中 ,8只作为正常对照组 ,其余 16只荷瘤后 ,随机分为两组 ,E coliDNA治疗组和TES对照组。荷瘤后第 4d予DNA治疗组、TES对照组小鼠皮下注射E coliDNA( 10 0 μg/ml)或TES ( 0 1mol/L) 0 3ml/只 ,隔日 1次 ,共 5次 ,荷瘤后第 2 1d ,摘眼球取血 ,制备血清 ,测定TNF -α、NO含量。结果显示 :E coliDNA可调节荷瘤机体的TNF -α、NO水平 ,防止TNF -α的异常增高及NO的显著下降 ,维持其在正常水平范围内 ,充分发挥其抗肿瘤免疫活性。这为细菌DNA作为一种新的生物反应调节剂提供了一些理论支持
Twenty-four BALB / c mice served as normal control group, and the remaining 16 mice were randomly divided into two groups: E coli DNA treatment group and TES control group. The mice in the TES control group were injected subcutaneously with E coli DNA (10 0 μg / ml) or TES (0 1 mol / L) 0 3 ml / once a day for 5 times after the tumor cells were treated with DNA. On the 21st day, blood was taken from the eye and serum was prepared to determine the content of TNF-α and NO. The results showed that: E coliDNA can regulate the level of tumor necrosis factor-α and tumor necrosis factor-α and prevent the abnormal increase of TNF-α and NO, and maintain its normal level of anti-tumor immune activity. This provides some theoretical support for bacterial DNA as a new biological response modifier