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神经系统不论在动物和人都是最复杂的结构,细胞种类多,数目大,相互种间关系很复杂。为进一步开展神经生物学的研究,神经组织体外培养技术便被选为一个简便和合适的方法。近年来国内或以实验动物的中枢和周围神经组织作体外培养的报道很多,并且开展了不少工作。可是值得提出的是:神经细胞培养得成功与否,虽然环节很多,但很大程度上和所取组织的部位与动物的年龄有关,这是一个关键性的问题。我们在多年的工作中积累了一些经验和体会,在此作一简短的介绍,为有志于神经组织培养者在开始工作时提供参考。神经组织培养成活的标志是:在分离细胞培养的标本中,当种植后24小时内贴壁,逐渐生长出几十微米的突起,神经细胞大多都能存活。器官培养的标本只要组织片薄,不飘浮,这就是贴壁,而后有生长晕
Nervous system in both animals and humans are the most complex structure, cell types and more, the number of large, inter-species relationship is very complicated. In order to further carry out the research of neurobiology, the nerve tissue culture technology was chosen as a simple and suitable method. In recent years, there are many reports about the in vitro culture of the central and peripheral nerve tissues in domestic or experimental animals, and a lot of work has been done. However, it is worth mentioning that the success or failure of nerve cell culture is a key issue, although it is related to the location of the tissues and the age of the animal to a large extent. We have accumulated some experience and experience in many years of work. We make a brief introduction here and provide a reference for those who are interested in nerve tissue culture. Nerve tissue culture is a sign of survival: in the separation of cell culture specimens, adherent to the wall within 24 hours after planting, and gradually grow tens of micron protrusions, most of the nerve cells can survive. Organ culture specimens as long as the tissue film is thin, not floating, which is adherent, and then grow halo