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目的:研究白介素18基因启动子多态性与儿童EV71感染遗传易感性的关系。方法:收集EV71感染患儿177例,单纯HFMD组127例,HFMD并脑炎组50例,提取外周血DNA,用序列特异性引物-聚合酶链反应(SSP-PCR)技术及基因测序法检测IL-18启动子区-137G/C、-607A/C位点的基因多态性。结果:EV71感染患儿与健康儿童IL-18-607基因型以CA为主,AA、CC次之,EV71感染患儿AA基因型、A等位基因分布频率显著高于健康儿童。EV71感染HFMD并脑炎组患儿AA基因型分布显著高于单纯HFMD组患儿,差异有统计学意义。EV71感染患儿与健康儿童IL-18-137基因型以CC为主,CG次之,GG占少数。该位点基因型及等位基因在EV71感染组与健康儿童、单纯HFMD与HFMD并脑炎组的分布无显著差异。结论:IL-18基因多态性与EV71感染相关,IL-18-607AA基因型、A等位基因携带儿童更易感染EV71病毒,且AA基因型患儿易并发脑炎,-607AA基因型可能为EV71感染的易感基因型。-137C/G位点基因多态性与EV71感染无相关性。
Objective: To investigate the relationship between genetic polymorphism of interleukin 18 gene and susceptibility to EV71 infection in children. Methods: Peripheral blood DNA was extracted from 177 children with EV71 infection, 127 from HFMD alone group and 50 from HFMD encephalitis group. The DNA of peripheral blood was collected and sequenced by sequence-specific primer-polymerase chain reaction (SSP-PCR) and sequencing IL-18 promoter region -137G / C, -607A / C locus gene polymorphisms. Results: The genotypes of IL-18-607 in children with EV71 infection and healthy children were mainly CA, followed by AA and CC. The frequency of AA genotype in AA children with EV71 infection was significantly higher than that in healthy children. The distribution of AA genotype in children with HFMD and encephalitis infected with EV71 was significantly higher than that in children with HFMD alone, the difference was statistically significant. The genotypes of IL-18-137 in children with EV71 infection and healthy children were mainly CC, followed by CG, which accounted for the minority. There were no significant differences in the genotypes and alleles between the EV71 infection group and healthy children, HFMD alone and HFMD encephalitis group. CONCLUSION: IL-18 gene polymorphism is associated with EV71 infection. Children with IL-18-607AA genotype and A allele are more likely to be infected with EV71 virus, and children with AA genotype are more likely to develop encephalitis. The -607AA genotype may be EV71 infection susceptibility genotypes. There was no correlation between -137C / G polymorphism and EV71 infection.