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目的探讨手工制备浓缩白细胞最佳离心条件。方法采用不同条件手工制备浓缩白细胞,测定不同离心条件下制备1 U浓缩白细胞计数占1 U全血计数百分比即收集率变化。结果组3、4收集率最高接近70%,高于组1、2、5、6组。组3、4与组1、2、5、6间收集率的差异有统计学意义(P<0.01)。而组1、2、5、6间收集率的差异无统计学意义(P>0.05)。组4为得到较高收集率的临界点。结论相对离心力2 515 g离心时间10 min条件下,制备浓缩白细胞最为合适且收集率最高。
Objective To investigate the optimal centrifugation conditions of manual preparation of concentrated leukocytes. Methods The white blood cells were prepared by hand under different conditions, and the percentage of collection rate of 1 U concentrated white blood cells (1 U whole blood) was calculated under different centrifugation conditions. Results The highest collection rate of groups 3 and 4 was close to 70%, higher than that of groups 1, 2, 5 and 6. There were significant differences in the collection rates between groups 3 and 4 and groups 1, 2, 5 and 6 (P <0.01). There was no significant difference in the collection rates between groups 1, 2, 5 and 6 (P> 0.05). Set 4 is the critical point to get a higher collection rate. Conclusion The relative centrifugal force of 2 515 g centrifugation time of 10 min, the most suitable preparation of concentrated white blood cells and the highest collection rate.