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目的 探讨新型基因工程人肿瘤坏死因子（ｎｒｈ Ｔ Ｎ Ｆ） 和足叶乙甙（ Ｖ Ｐ－ １６） 的协同抗肿瘤作用及ｎｒｈ Ｔ Ｎ Ｆ 的毒副作用。方法 以复制成功的 Ｌｅｗｉｓ 肺癌（３ Ｌ Ｌ） 小鼠为模型局部用药。结果 ｎｒｈ Ｔ Ｎ Ｆ 或 Ｖ Ｐ－ １６ 瘤体内注射均能使肿瘤生长及肺转移受到一定的抑制，抑瘤率分别为３３７１ ％ 、３０４６ ％ ，肺转移瘤数与对照组比较 Ｐ ＜ ００５ ，肿瘤出现一定程度坏死。而联合用药则能显著抑制肿瘤生长及肺转移，抑瘤率为６５７７ ％ ，大于理论抑瘤率５３９１ ％ ，肺转移瘤数与对照组比较 Ｐ ＜ ００１ ，肿瘤出现广泛的出血坏死。同时观察到ｎｒｈ Ｔ Ｎ Ｆ 对实验小鼠无明显毒副作用。结论 ｎｒｈ Ｔ Ｎ Ｆ 和 Ｖ Ｐ－ １６联合应用，具有协同抗肿瘤作用，对于肺癌治疗具有进一步临床应用探讨价值。目的 探讨新型基因工程人肿瘤坏死因子（ｎｒｈ Ｔ Ｎ Ｆ） 和足叶乙甙（ Ｖ Ｐ－ １６） 的协同抗肿瘤作用及ｎｒｈ Ｔ Ｎ Ｆ 的毒副作用。方法 以复制成功的 Ｌｅｗｉｓ 肺癌（３ Ｌ Ｌ） 小鼠为模型局部用药。结果 ｎｒｈ Ｔ Ｎ Ｆ 或 Ｖ Ｐ－ １６ 瘤体内注射均能使肿瘤生长及肺转移受到一定的抑制，抑瘤率分别为３３７１ ％ 、３０
Objective To investigate the synergistic anti-tumor effects of novel gene-engineered human tumor necrosis factor (nrh T N F) and etoposide (V P-16) and the toxic side effects of nrh T N F. Methods A model of Lewis lung cancer (3 L L) mice successfully replicated was administered topically. Results Intratumoral injection of nrh T N F or V P-16 could inhibit tumor growth and lung metastasis. The tumor inhibition rates were 33.7%, 30.4%, respectively. Pulmonary metastases were compared with the control group. < 005, the tumor appeared necrotic to a certain extent. The combined use of drugs can significantly inhibit tumor growth and lung metastasis, the tumor inhibition rate was 65.77%, greater than the theoretical tumor inhibition rate of 53.91%, the number of lung metastases compared with the control group P <0. 01, the tumor appeared extensive. Bleeding and necrosis. At the same time, it was observed that nrh T N F has no obvious toxic side effects on experimental mice. Conclusion The combination of nrh T N F and V P-16 has a synergistic anti-tumor effect, and has further clinical application value for the treatment of lung cancer. Objective To investigate the synergistic anti-tumor effects of novel gene-engineered human tumor necrosis factor (nrh T N F) and etoposide (V P-16) and the toxic side effects of nrh T N F. Methods A model of Lewis lung cancer (3 L L) mice successfully replicated was administered topically. Results The intratumoral injection of nrh T N F or V P-16 could inhibit tumor growth and lung metastasis. The tumor inhibition rates were 3371 % and 30 respectively.