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目的:探讨orexin-A(OXA)受体介导的生长抑素激动剂ODT8-SST对大鼠摄食和饮水的调节作用相关作用机制。方法:在光照周期内,大鼠40只随机分8组,侧脑室(icv)分别注射不同剂量ODT8-SST或生理盐水(NS);大鼠56只随机分8组分别侧脑室注射不同剂量OXA受体(OX1R)拮抗剂SB-334867或NS;2小时后测量大鼠摄食量和饮水量。结果:与NS组相比,实验组大鼠侧脑室注射ODT8-SST(1μg/rat),2小时后摄食量和饮水量均显著增加(P<0.05)。大鼠侧脑室注射SB-334867(16μg/rat)完全抑制了由侧脑室注射ODT8-SST后引起的摄食量和饮水量的增加;与此相反,大鼠给予SST2拮抗剂S-406-028预处理之后,可阻止侧脑室注射ODT8-SST引发的促进食欲作用,但不会影响侧脑室注射OXA(10.7μg/rat)诱导的摄食量和饮水量的增加。结论:侧脑室注射ODT8-SST可促进摄食和饮水,该过程可能由OX1R所介导;orexin-A促进摄食作用不依赖大脑SST2通路的激活。
AIM: To investigate the role of orexin-A (OXA) receptor-mediated somatostatin agonist ODT8-SST in the regulation of ingestion and drinking water in rats. Methods: Forty rats were randomly divided into 8 groups: icv injection of ODT8-SST or NS; 56 rats were randomly divided into 8 groups and were given different doses of OXA Receptor (OX1R) antagonist SB-334867 or NS; food intake and water intake of rats were measured after 2 hours. Results: Compared with NS group, ODT8-SST (1μg / rat) was injected into the lateral ventricle of rats in experimental group, and food intake and water intake increased significantly after 2 hours (P <0.05). Intranal intracerebroventricular injection of SB-334867 (16 μg / rat) completely inhibited the increase in food intake and water intake induced by lateral ventricle injection of ODT8-SST; in contrast, rats were administered the SST2 antagonist S-406-028 pre- After treatment, it prevented the ODT8-SST-induced appetite-inducing effect in the lateral ventricle but did not affect the increase in food intake and water intake induced by intracerebroventricular injection of OXA (10.7μg / rat). Conclusion: Intracerebroventricular injection of ODT8-SST can promote food intake and water intake, which may be mediated by OX1R. Overexpression of orexin-A does not depend on the activation of brain SST2 pathway.