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目的:比较四君子汤配方颗粒与传统汤剂中总皂苷和总多糖含量的差异。方法:采用紫外分光光度法在540 nm、488 nm波长处分别对四君子汤配方颗粒与传统汤剂中总皂苷和总多糖的含量进行测定。结果:人参皂苷Re在10.909~65.454μg·ml~(-1)浓度范围内与吸光度呈良好的线性关系(r=0.9997),加样回收率为98.49%,RSD为0.85%(n=6);D-无水葡萄糖在2.160~12.960μg·ml~(-1)浓度范围内与吸光度呈良好的线性关系(r=0.9997),加样回收率为99.46%,RSD为0.73%(n=6)。3个批次的配方颗粒供试品溶液中总皂苷的含量略高于传统汤剂,而总多糖的含量略低于传统汤剂,两者含量差异无统计学意义(P>0.05)。结论:四君子汤配方颗粒与传统汤剂间的物质基础差异较小,该配方颗粒应用于临床具有一定的可行性。
OBJECTIVE: To compare the differences of total saponins and total polysaccharides in Sijunzitang formula granules and traditional decoctions. Methods: The contents of total saponins and total polysaccharides in Sijunzi decoction granules and traditional decoctions were measured by ultraviolet spectrophotometry at 540 nm and 488 nm respectively. Results: Ginsenoside Re showed a good linear relationship with absorbance (r = 0.9997) in the range of 10.909 ~ 65.454μg · ml ~ (-1), with a recovery of 98.49% and a RSD of 0.85% (n = 6) (R = 0.9997), the recovery rate was 99.46%, the RSD was 0.73% (n = 6), the linear range of D-glucose was 2.160-12.960μg · ml -1 ). The content of total saponins in the three batches of the test solution was slightly higher than that of the traditional decoction, while the content of total polysaccharides was slightly lower than that of the traditional decoction. There was no significant difference between the two groups (P> 0.05). Conclusion: The difference of material basis between Sijunzi decoction formula and traditional decoction is small. It is feasible for the formula particle to be used clinically.