论文部分内容阅读
目的:探讨致炎剂联合降钙素基因相关肽对大鼠硬脑膜神经源性炎症的影响,寻求最佳慢性偏头痛的实验模型。方法:24只SD雄性大鼠,随机分为生理盐水组(NS)、降钙素基因相关肽组1(CGRP 1,10-3M)、降钙素基因相关肽组2(CGRP 2,10-4M),以及IS(20μL)+CGRP(10μL,10-4M)组,每组各6只,通过甲苯胺蓝染色法,观察肥大细胞脱颗粒,采用伊文氏蓝荧光显像法,观察大鼠硬脑膜血管渗出,采用多普勒激光血流仪检测法,观察各组硬脑膜动脉血流量变化。结果:与NS组相比,CGRP1、CGRP2、IS+CGRP组肥大细胞脱颗粒数和比率、脑血流量均明显升高,P<0.05,并且硬脑膜荧光红斑明显增多;与CGRP1、CGRP2组相比,IS+CGRP组肥大细胞脱颗粒数和比率、脑血流量均明显升高,P<0.05,并且硬脑膜荧光红斑明显增多。CGRP两组相比,上述指标比较,差异没有统计学意义,P>0.05。结论:IS+CGRP能够明显刺激大鼠硬脑膜发生神经源性炎症反应,可以作为慢性偏头痛动物实验模型。
Objective: To investigate the effect of inflammatory cytokines and calcitonin gene-related peptide on dural neurogenic inflammation in rats and to find the best experimental model of chronic migraine. Methods: Twenty-four SD male rats were randomly divided into four groups: NS, CGRP1,10-3M, CGRP2,10- 4M), and IS (20μL) + CGRP (10μL, 10-4M) group, 6 in each group. Toluidine blue staining was used to observe mast cell degranulation. Dural vascular exudation, using Doppler laser flowmeter detection method to observe the changes in the dural artery blood flow in each group. Results: Compared with NS group, the degranulation rate and cerebral blood flow of mast cells in CGRP1, CGRP2 and IS + CGRP groups were significantly increased (P <0.05), and the number of erythema of dura in the CGRP1, CGRP2 and IS + Compared with IS + CGRP group, the degranulation number and ratio of mast cells and cerebral blood flow were significantly increased (P <0.05), and the number of fluorescent erythema in dura was significantly increased. CGRP compared to the two groups, the above indicators, the difference was not statistically significant, P> 0.05. Conclusion: IS + CGRP can significantly stimulate neurogenic inflammation in rat dura mater and can be used as an experimental animal model of chronic migraine.