OBJECTIVE:To investigate the inhibitive efficacy of Nymphoides Indica(L.)Kuntze rhizome extract onα-glucosidase and on cross-link formation of ad-vanced glycation end products(AGEs).METHODS:The plant extracts were prepared by cold maceration and fractionated in solvents of di-verse polarity.The in vitro α-glucosidase inhibition assay,fluorescence spectrometry and SDS-PAGE analysis was performed for antiglycation assays.RESULTS:During α-glucosidase inhibition assay significant inhibition by chloroform(0.43 mg/mL)and methanol fractions(0.66 mg/mL)was noticed.During the AGEs inhibition assay,both oxidative(BSA-MGO)and non-oxidative(BSA-glucose)modes were employed.The inhibition of AGEs by total extract was considered moderate(IC50 0.10 mg/mL)as a result of non-oxidative mode,whereas in case of oxidative mode(BASA-MGO)no activity was recorded.Among fractions the methanolic frac-tion presented significant results both in oxidative(IC50 0.01 mg/mL)and non-oxidative modes(IC500.3 mg/mL).Likewise the ethyl acetate fraction was more active in non-oxidative mode(IC50 0.04 mg/mL)compared to oxidative mode(IC50 0.32 mg/mL).During assay for inhibition of cross-link formation,the chloroform fraction significantly inhibited cross-link formation in a dose dependent mode.CONCLUSION:It was finally concluded that N.Indi-ca rhizome extract possesses significant properties that inhibit α-glucosidase,and AGEs cross-link for-mation.