Growth and radiosensitivity of irradiated human glioma cell progeny

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BACKGROUND: Progenitors of the immortalized human glioma cell line, SHG-44, are significantly less sensitive to irradiation. Two hypotheses regarding the mechanism of this effect exist: several studies have suggested that there is a subgroup with different radiosensitivities in identical cell group, and the progenitors of irradiate is a adaptive response subgroup, so its radiosensitivity is descend. A second hypothesis suggests that irradiated glioma progeny have a stronger ability to repair DNA damage. This would suggest that when progeny are continuously irradiated, resistance to irradiation-induced DNA increases, and radiosensitivity decreases. OBJECTIVE: To investigate radiosensitivity and growth features after irradiation to progeny of the human glioma cell line SHG-44. DESIGN, TIME AND SETTING: A randomized, controlled experiment, which was performed at the Department of Radiology Laboratory, the First Hospital Affiliated to Soochow University, between September 2004 and January 2006. MATERIALS: The glioma cell line SHG-44 was provided by the Institute of Neuroscience, First Affiliated Hospital of Suzhou University. Propidium iodide reagent was provided by Coulter Corporation. A linear accelerator, KD-2 type, was provided by Siemens, Germany. The flow cytometer EPICS-XL was provided by Coulter Corporation. METHODS: Brain glioma SHG-44 cells were divided into four groups: SHG-44, SHG-44-2, SHG-44-6, and SHG-44-10 . The SHG-44-2, SHG-44-6, and SHG-44-10 cells were vertically irradiated with varying doses of 2, 6 and 10 Gy by a linear accelerator (6 MVX). The cells were passaged for 15 generations and cultured in RPMI-1640 culture media. MAIN OUTCOME MEASURES: Community re-double time, mean lethal dose (D0), extrapolation number (N), fraction surviving fraction irradiated by 2 Gy dose (SF2), quasi-threshold dose (Dq), and cell cycle. RESULTS: The Population doubling time (PDT) of SHG-44-2, SHG-44-6, and SHG-44-10 cell groups was not significant (P = 0.052). Compared to these three groups, the PDT of the SHG-44 cell group was significantly difference (F = 7.878, P < 0.002). SHG-44 cell clone ratewas 26.5%, and SHG-44-10 cell group was 15.5%. The SHG-44-10 cell group also exhibited radiosensitivity, but was less than the radiosensitivity of the SHG-44 cell group. Compared to the SHG-44 cell group, the ratio of the G2/M phase was decreased in the SHG-44-10 cell group, and the radio of S phase was increased. The SHG-44 and SHG-44-10 cell groups were irradiated with 8 Gy. After 12 hours, the G2/M ratio was compared to pre-irradiation times, indicating a significantly higher ratio in the pre-irradiated groups (P < 0.01). The cells between SHG-44 and SHG-44-10 groups were harvested 12 hours after irradiation: G2 phase of SHG-44-10 cells was arrested and the G2/M ratio was increased, which was intensified with increasing irradiation doses. CONCLUSION: In the present study, the proliferation delay and decreased radiosensitivity were confirmed in progeny of irradiated human glioma cells, and radiosensitivity was dose-dependent. BACKGROUND: Progenitors of the immortalized human glioma cell line, SHG-44, are significantly less sensitive to irradiation. Two hypotheses regarding the mechanism of this effect exist: several studies have suggested that there is a subgroup with different radiosensitivities in identical cell group, and The progenitors of irradiate is an adaptive response subgroup, so its radiosensitivity is descend. A second hypothesis that irradiating DNA damage. This would suggest that when progeny are continuously irradiated, resistance to irradiation-induced DNA damage. , and radiosensitivity decreases. OBJECTIVE: To investigate radiosensitivity and growth features after irradiation to progeny of the human glioma cell line SHG-44. DESIGN, TIME AND SETTING: A randomized, controlled experiment, which was performed at the Department of Radiology Laboratory, the First Hospital Affiliated to Soochow University, between September 2004 and January MATERIALS: The glioma cell line SHG-44 was provided by the Institute of Neuroscience, First Affiliated Hospital of Suzhou University. Propidium iodide reagent was provided by Coulter Corporation. A linear accelerator, KD-2 type, was provided by Siemens, Germany . The flow cytometer EPICS-XL was provided by Coulter Corporation. METHODS: Brain glioma SHG-44 cells were divided into four groups: SHG-44, SHG-44-2, SHG-44-6, and SHG-44-10. The SHG-44-2, SHG-44-6, and SHG-44-10 cells were vertically irradiated with varying doses of 2, 6 and 10 Gy by a linear accelerator (6 MVX). The cells were passaged for 15 generations and culture in RPMI-1640 culture media. MAIN OUTCOME MEASURES: Community re-double time, mean lethal dose (D0), extrapolation number (N), fraction surviving fraction irradiated by 2 Gy dose (SF2) , and cell cycle. RESULTS: The Population doubling time (PDT) of SHG-44-2, SHG-44-6, and SHG-44-10 cell groups was not significant (P = 0.052)The SHG-44 cell clone rate was 26.5%, and SHG-44-10 cell group was 15.5%. The red to these three groups, the PDT of the SHG- 44 cell group was significantly worse (F = 7.878, P <0.002) Compared to the SHG-44 cell group, the ratio of the G2 / M phase was decreased in the SHG-44 cell group. Compared to SHG- 10 cell group, and the radio of S phase was increased. The SHG-44 and SHG-44-10 cell groups were irradiated with 8 Gy. After 12 hours, the G2 / M ratio was compared to pre-irradiation times, indicating a The cells between SHG-44 and SHG-44-10 groups were harvested for 12 hours after irradiation: G2 phase of SHG-44-10 cells was arrested and the G2 / M ratio was increased, which was intensified with increasing irradiation doses. CONCLUSION: In the present study, the proliferation delay and decreased radiosensitivity were confirmed in progen y of irradiated human glioma cells, and radiosensitivity was dose-dependent.
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