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目的 :探讨小剂量 3 硝基丙酸 (3 nitropropionicacid ,3 NPA)预处理诱导沙土鼠脑缺血耐受的可行性。方法 :将 5组沙土鼠腹腔分别注射双蒸水 5mg/kg、10mg/kg、15mg/kg、2 0mg/kg 3 NPA ,于注射 3 NPA后 3d ,透射电镜观察纹状体神经元细胞核和细胞器超微结构形态的变化 ;每组均分别于注射后 1d ,2d ,3d ,4d阻断沙土鼠双侧颈总动脉造成前脑缺血模型 ,恢复血流期满时处死动物 ,通过CresylViolet染色观察海马锥体细胞层存活神经元数目结果 :应用不同剂量 3 NPA后 3d ,纹状体神经元细胞核均无明显变化 ,腹腔注射 15mg/kg及 2 0mg/kg 3 NPA组线粒体和粗面内质网轻度肿胀。应用不同剂量 3 NPA 1～ 3d后 ,前脑缺血沙土鼠海马CA1区锥体细胞层存活神经元数目均高于双蒸水对照组 (P <0 .0 1或 0 .0 5 ) ,第 4d则下降 ,与双蒸水对照组比较差异无统计学意义 (P >0 .0 5 )。结论 :应用小剂量 3 NPA诱导脑缺血耐受较安全 ,具有时间依赖性 ,无剂量依赖性
Objective: To investigate the feasibility of 3-nitropropionic acid (3-NPA) preconditioning to induce cerebral ischemic tolerance in gerbils. Methods: 5 mg / kg, 10 mg / kg, 15 mg / kg and 20 mg / kg of 3 NPA were intraperitoneally injected into the peritoneal cavity of 5 groups of gerbils respectively. After 3 NPA injection for 3 days, the nuclei and organelles of neurons in the striatum were observed by transmission electron microscope The morphological changes of the ultrastructure were observed in each group. The forebrain ischemia model was induced on the bilateral common carotid arteries in gerbils 1d, 2d, 3d and 4d after injection respectively. At the expiration of blood flow recovery, animals were sacrificed and observed by Cresyl Violet staining The number of neurons in hippocampal pyramidal cell layer. RESULTS: Three days after administration of 3 NPA, there was no significant change in nuclei of striatum neurons. Intraperitoneal injection of mitochondria and rough endoplasmic reticulum at 15 mg / kg and 20 mg / kg 3 NPA Mild swelling. The number of neurons in pyramidal cell layer of hippocampal CA1 pyramidal neurons in forebrain hippocampus was significantly higher than that in double distilled water control group (P <0.01 or 0.55) after application of different doses of 3 NPA for 1-3 days. 4d decreased, compared with double distilled water control group no significant difference (P> 0.05). Conclusion: It is safe, time-dependent and dose-independent