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目的 探讨神经再生过程中细胞骨架蛋白的作用机制 ,了解中枢和外周神经系统神经元应答神经损伤和再生的差异。 方法 用原位分子杂交方法观察了大鼠T10～ 11脊髓半横断损伤后 1、3、5、7、10、14、2 1、2 8、5 6d皮层感觉运动区皮层神经元中α 管蛋白和 3个神经丝蛋白亚基mRNA的表达变化。 结果 脊髓半横断损伤后损伤侧皮层感觉运动区皮层神经元中α 管蛋白、神经丝 L、 M、 HmRNA表达明显下调。α 管蛋白mRNA表达水平在损伤后 1d降低 ,而神经丝亚基mRNA表达水平直到下一个时间点 (损伤后 3d)才下调 ,至损伤后 5 6d均无恢复趋势。结论 皮层神经元损伤后α 管蛋白mRNA的表达被抑制 ,提示皮层神经元和外周神经元应答损伤的信号不同
Objective To investigate the mechanism of cytoskeletal protein during the process of neural regeneration and to understand the differences between the neurons in central and peripheral nervous system in response to nerve injury and regeneration. Methods In situ hybridization was used to observe the expression of α-tubulin in the cortical neurons of cortical sensory zone of rats at 1, 3, 5, 7, 10, 14, And three neurofilament subunit mRNA expression changes. Results The expressions of α-tubulin, L, M and H mRNA in cortical neurons of cortical sensory zone decreased significantly after spinal cord transection. The expression of α-tubulin mRNA decreased at 1 day after injury, while the expression of neurofilament subunit mRNA was not down-regulated until the next time point (3d after injury), and showed no trend of recovery at 56 days after injury. Conclusion The expression of α-tubulin mRNA is inhibited after cortical neuron injury, suggesting that the signal of cortical neurons and peripheral neurons responding to injury is different