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目的:检测Hedgehog信号通路基因PTCH-1和SMO在鼻咽癌(NPC)组织中的表达及其生物学意义。方法:利用实时荧光定量PCR分别检测32例鼻咽癌组织及32例鼻咽炎组织中PTCH-1和SMO基因的表达情况。根据Livak(2-△△Ct)法进行相对定量表达分析,以鼻咽炎组织为对照,计算鼻咽癌组织中PTCH-1和SMO表达水平。结果:在所检测的全部鼻咽癌组织和鼻咽炎组织中PTCH-1和SMO都有表达。与鼻咽炎组织相比,有75%(24/32)鼻咽癌组织的PTCH-1基因表达水平下调,有68.8%(22/32)鼻咽癌组织的SMO表达水平上调,差异均具有统计学意义(P<0.05)。此外,有18.7%(6/32)鼻咽癌组织PTCH-1基因表达水平上调,有15.6%(5/32)鼻咽癌组织SMO基因表达水平下调,差异均无统计学意义(P>0.05)。结论:在鼻咽癌中SMO基因普遍具有较高的表达水平,PTCH-1基因的表达水平则相对较低,而SMO基因表达水平的上调及PTCH-1基因表达水平的下调,可引起Hedgehog信号通路在鼻咽癌中的异常激活,Hedgehog信号通路的异常激活可能与鼻咽癌的发生发展过程相关。
Objective: To detect the expression of Hedgehog signaling pathway genes PTCH-1 and SMO in nasopharyngeal carcinoma (NPC) and their biological significance. Methods: The expression of PTCH-1 and SMO genes in 32 cases of nasopharyngeal carcinoma and 32 cases of nasopharyngitis were detected by real-time fluorescence quantitative PCR. The expression of PTCH-1 and SMO in nasopharyngeal carcinoma were calculated according to Livak (2- △△ Ct) method and nasopharyngeal tissue as control. Results: PTCH-1 and SMO were detected in all nasopharyngeal carcinoma tissues and nasopharyngeal tissues. Compared with nasopharyngitis tissues, the expression of PTCH-1 gene in 75% (24/32) of nasopharyngeal carcinoma tissues was down-regulated, and 68.8% (22/32) of NPC tissues showed an up-regulation of SMO expression, with statistical differences Significance (P <0.05). In addition, the expression of PTCH-1 was up-regulated in 18.7% (6/32) of nasopharyngeal carcinomas, and the expression of SMO was down-regulated in 15.6% (5/32) of nasopharyngeal carcinomas, with no significant difference (P> 0.05 ). CONCLUSIONS: SMO gene is generally highly expressed in nasopharyngeal carcinoma and PTCH-1 gene expression is relatively low. However, up-regulation of SMO gene expression and down-regulation of PTCH-1 gene expression can cause Hedgehog signaling Pathway abnormal activation in nasopharyngeal carcinoma, Hedgehog signaling pathway abnormal activation may be associated with the occurrence and development of nasopharyngeal carcinoma.