论文部分内容阅读
目的 调查一组耐碳青霉烯类肺炎克雷伯菌(Carbapenem-resistant Kebsiellal pneumoniae,CRKP)β-内酰胺酶基因的存在状况。方法 收集分离自2016年某医院ICU的20株CRKP菌;采用K-B纸片扩散法进行17种药物敏感性判断,采用聚合酶链反应(PCR)法检测A、B、C、D四类36种β-内酰胺酶基因,PCR法连锁检测KPC型β-内酰胺酶编码基因与插入序列(KPC-ISKpn6)。结果 20株CRKP菌每株均检出bla TEM、bla SHV、bla KPC和bla NDM型β-内酰胺酶基因,阳性率分别为100.00%、100.00%、75.00%、100.00%;15株KPC阳性菌株KPC-ISKpn6连锁检测均为阳性。结论 bla TEM、bla SHV、bla KPC、bla NDM等4种基因的菌株在该组CRKP菌中流行;KPC型β-内酰胺酶编码基因由插入序列ISKpn6介导。
Objective To investigate the presence of a group of β-lactamase genes that are resistant to carbapenem-resistant Kebsiella pneumoniae (CRKP). Methods Twenty strains of CRKP isolated from ICU in a hospital in 2016 were collected. Seventeen drug sensitivity tests were carried out using KB disk diffusion method. Thirty-six strains of A, B, C and D were detected by polymerase chain reaction (PCR) β-lactamase gene and PCR method to detect KPC β-lactamase encoding gene and insert (KPC-ISKpn6). Results The positive rates of bla TEM, bla SHV, bla KPC and bla NDM were 20.00%, 100.00%, 75.00% and 100.00% respectively in 20 strains of CRKP. 15 strains of KPC positive KPC-ISKpn6 chain test are positive. Conclusion The strains of bla TEM, bla SHV, bla KPC and bla NDM are the most common strains in this group of CRKP. The KPC β-lactamase-encoding gene is mediated by ISKpn6.