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目的探讨流式细胞术双标记法检测恶性实体肿瘤细胞ＤＮＡ含量、细胞周期和Ｋｉ６７表达和组织学分级之间的关系。方法利用流式细胞术Ｋｉ６７／ＤＮＡ双标记法同时检测８７例新鲜恶性实体肿瘤的ＤＮＡ含量、细胞周期和增殖核抗原Ｋｉ６７的表达。结果异倍体发生率为４０．２％，其中高分化肿瘤为１１．ｌ％，中分化肿瘤为３７．５％，低分化肿瘤为 ４６． ３％。 Ｋｉ６７阳性细胞为 ０． ５％～８７％（１７． ３６％士１６． ６％）。 Ｓ期细胞百分比为 ０～２４％（５ ２８％±４． ８５％）。高分化肿瘤 Ｓ期细胞百分比及增殖核抗原 Ｋｉ６７的表达明显低于中分化和低分化肿瘤，统计学上有显著性差异（Ｐ＜０．０１）。中分化和低分化肿瘤之间则差异无显著性（Ｐ＞０．０５）。异倍体肿瘤Ｓ期细胞百分比高于二倍体肿瘤，差异有显著性（Ｐ＜０．０１），而Ｋｉ６７的表达在两者之间无显著性差异。结论流式细胞术Ｋｉ６７／ＤＮＡ双标记法可同时检测实体恶性肿瘤细胞的ＤＮＡ含量、细胞周期和增殖核抗原Ｋｉ６７的表达，并能进一步阐明这些参数与组织学分级的关系。方法简便、快速，有利于对肿瘤生物学特性的了解。
Objective To investigate the relationship between DNA content, cell cycle, Ki67 expression and histological grade in malignant solid tumor cells by flow cytometry double labeling. Methods Flow cytometric Ki67/DNA dual-labeling method was used to detect the DNA content, cell cycle and expression of proliferating nuclear antigen Ki67 in 87 cases of fresh solid tumors. The incidence of aneuploidy was 40.2%, of which the highly differentiated tumor was 11. l%, moderately differentiated tumors were 37.5%, poorly differentiated tumors were 46. 3%. Ki67 positive cells are 0. 5% to 87% (17.36% persons 16.6%). The percentage of cells in S phase was 0-24% (5 28%±4.85%). The percentage of S-phase cells and the expression of proliferating nuclear antigen Ki67 in highly differentiated tumors were significantly lower than those in moderately differentiated and poorly differentiated tumors, with statistically significant differences (P<0.01). There was no significant difference between moderately differentiated and poorly differentiated tumors (P>0.05). The percentage of S-phase cells in aneuploid tumors was higher than that of diploid tumors, and the difference was significant (P<0.01). There was no significant difference in the expression of Ki67 between the two groups. Conclusion Flow cytometry Ki67/DNA double-labeling method can simultaneously detect DNA content, cell cycle and expression of proliferating nuclear antigen Ki67 in solid malignant cells, and can further clarify the relationship between these parameters and histological grade. The method is simple, rapid, and conducive to understanding the biological characteristics of tumors.