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免疫金银染色(IGSS)是一较新而灵敏的免疫组织化学方法,近来我们对多年累积的27例流行性出血热(下称流热)尸检的病变组织蜡块,重新进行常规切片厚4um,应用直接法与间接法免疫金银染色,(方法与观察结果见潍坊医学院学报1989年第2期)定位检测流热组织的抗原,结果认为是值得推广的一种免疫组化技术。现将此染色法的优点与注意事项分别介绍如下: 一、优点 1、IGSS法较为敏感与可靠本法做为细胞化学的标记物,在国内近几年才开始应用,而对流热抗原进行标记,国内还未有报告。由于尸检组织陈旧,又经石蜡制片过程,抗原破坏较多,在数种免疫组化的方
Immune gold and silver staining (IGSS) is a relatively new and sensitive method of immunohistochemistry, recently we have accumulated 27 years of epidemic hemorrhagic fever (hereinafter referred to as fluid heat) autopsy lesions of paraffin blocks, re-conventional section thickness 4um , The application of direct and indirect immunostaining silver staining (methods and observations, see Weifang Medical College in 1989 the second period) to detect the flow of heat tissue antigen, the results considered worthy of promotion of an immunohistochemical technique. Now the advantages and precautions of this staining method are described as follows: First, the advantages 1, IGSS method is more sensitive and reliable This method as a marker of cytochemistry in China in recent years began to apply, and labeling of convection heat antigen , There is no domestic report. As the autopsy organization obsolete, but also paraffin preparation process, antigen destruction more in the number of immunohistochemical side