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本文仅对心脏手术中缺血,重获氧和血灌注所造成的再灌注损伤的现象进行了研究。用硝酸镧作示踪剂,检查了心肌细胞膜通透性的改变。对正常细胞,只能存在于细胞膜外,而在膜受损后则可以进入细胞内,置换结合钙,故可作为缺血心肌细胞膜受损和通透性增加的标记。电镜图像显示出:正常组镧颗粒沉积在闰盘处,细胞内细胞器上则没有;缺氧组由于细胞膜损坏,通透性增强,闰盘处沉积物减少,形成一条稀疏带,而线粒体膜上却出现了镧的沉积物。表明用硝酸镧示踪法研究心肌缺氧等损伤条件下细胞膜通透性的改变是较好的方法。
This article focuses on the phenomenon of ischemia-reperfusion and reperfusion injury caused by blood perfusion in cardiac surgery. Lanthanum nitrate was used as a tracer to examine changes in membrane permeability of the myocardial cells. Right normal cells, can only exist in the cell membrane, and after the membrane is damaged you can enter the cells, replacing the combination of calcium, it can be used as ischemic myocardial cell membrane damage and increased permeability markers. Electron microscopy images showed that normal group lanthanum particles deposited on the intercalated disk but not on intracellular organelles. The hypoxia group formed a sparse band due to cell membrane damage and increased permeability, and reduced the deposits at the intercalated disk, whereas the mitochondrial membrane But there lanthanum deposits. It is a good method to study the change of cell membrane permeability under the condition of hypoxia and other damage by using lanthanum nitrate tracing method.