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为了解乙型脑炎 (乙脑 )减毒活疫苗弱毒株SA14 14 2神经毒力的减弱程度 ,本文对弱毒株及其原株SA14强毒株进行了猴体和小白鼠的致病性和病理学变化的比较试验。SA14强毒株病毒 (原始滴度 6 15× 10 8/ml) ,以10 -2 和 10 -4 ～ 10 -7不同稀释度于丘脑两侧合并脊髓注射恒河猴 ,每组除 10 -4 1只外其余均为 2只。另以 10 -4 和10 -6～ 10 -8不同稀释度脑内注射小鼠 ,每组 8只。结果猴子除 10 -4 1只外其余全部发病死亡 ,小鼠则全部死亡。SA14 14 2以 1∶5稀释病毒 (原始滴度为 8× 10 6/ml)按同样方法注射 4只猴和 30只小鼠 ,结果全部存活。另以SA1410 -2 病毒皮下注射 3只猴未死亡 ,而以 10 -1皮下注射 30只小鼠时则全部死亡。SA14 14 2以 1∶5稀释病毒皮下注射小鼠时则全部存活。病理组织学结果显示二种动物接种SA14株强毒后主要表现为弥散性脑脊髓炎 ,以神经细胞坏死为其主要特征和最突出的病变。猴子的病变以脊髓前角、丘脑和中脑黑质为重 ,小鼠的病变则以大脑皮质、海马部最重 ,脊髓的病变却比脑轻。接种弱毒株的动物则仅有轻微炎症反应、神经细胞坏死极少出现。以上结果表明以脑内接种时恒河猴和小鼠对乙脑病毒均高度敏感 ,以皮下接种时小鼠的敏感性高于猴子。乙脑SA14 14 2弱毒株的神经毒力包括致
In order to understand the attenuating degree of neurotoxicity of attenuated SA14 14 2 attenuated live attenuated encephalitis virus (JE), the pathogenicity of the attenuated SA14 virulent strain and its virulent SA14 virulent strain Comparison of pathological changes in the test. SA14 virulent strain of virus (original titer of 6 15 × 10 8 / ml), 10 -2 and 10 -4 to 10 -7 different dilutions in the thalamus on both sides of the spinal cord injection of rhesus monkeys, each group in addition to 10 -4 1 only the rest are two. Another 10 -4 and 10 -6 ~ 10 -8 different dilutions of intracerebral injection of mice, each group of eight. Results In addition to 10 -4 1 monkeys except the rest all the disease died, all mice died. SA14 14 2 The virus was diluted 1: 5 (original titers of 8 × 10 6 / ml) Four monkeys and 30 mice were injected in the same manner and all survived. Another SA1410 -2 virus subcutaneous injection of three monkeys did not die, but to 10 -1 subcutaneous injection of 30 mice all died. SA14 14 2 all survived when mice were subcutaneously injected with a 1: 5 dilution of virus. Histopathological results showed that SA14 strains inoculated with two kinds of animals showed diffuse encephalomyelitis after their virulent virulence. The main characteristics and the most prominent lesions were neurofibrillary necrosis. The lesions of the monkey were characterized by spinal cord anterior horn, thalamic and midbrain substantia nigra, and the lesions of mice were the heaviest in the cerebral cortex and hippocampus. The lesion in the spinal cord was lighter than that in the brain. Animals vaccinated with attenuated strains had only a slight inflammatory response, and neuronal cell death was rare. The above results indicate that rhesus monkeys and mice are highly sensitive to Japanese encephalitis virus when inoculated intracerebrally, and are more sensitive than monkeys for subcutaneous vaccination. Encephalitis SA14 14 2 Virulence of virulent strains Including induced