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以胶原和肾上腺素混合液给小鼠 ,制造肺血栓模型 ,用放免法测定 6-酮 -前列腺素F1α ( 6-keto_PGF1α)和血栓素B2 (TXB2 )含量。结果表明 ,花椒油素 (Xanthoxylin ,XT ) 1 .0mg/kg,2 .0mg/kg,4 .0mg/kg和 8.0mg/kg使小鼠的存活率增加 2 8.6%～ 71 .2 % ;使 6_keto_PGF1α的产量提高 56.3 %～ 2 1 0 .8% ,使TXB2 的产量降低 2 1 .6%～ 56.8%。 6_keto_TGF1α与TXB2 的比值明显提高 ,对照组为 1 .1 5,药物组为2 .2 9,3 .1 3 ,5.53 ,8.2 6。体外实验证明 ,XT 0 .0 3 7μmol/L,0 .1 85μmol/L,0 .92 4 μmol/L,9.2 4 0 μmol/L显著地抑制血小板聚集和TXB2 产生 ,前者的抑制率为 2 2 .7%～ 55.3 % ,后者为 2 0 .5%～ 50 .7% ;但同时增加 6_keto_PGF1α的产量 ,提高率为 2 7.3 %～ 4 7.0 %。 6_keto_PGF1α与TXB2 比值 :对照组为 1 .2 3 ,药物组为2 .0 7,2 .4 4,3 .2 8,3 .85。
A mixture of collagen and epinephrine was administered to mice to establish a pulmonary thrombosis model. The levels of 6-keto-prostaglandin F1α (6-keto_PGF1α) and thromboxane B2 (TXB2) were determined by radioimmunoassay. The results showed that xanthoxylin (XT) of 1.0 mg/kg, 2.0 mg/kg, 4.0 mg/kg, and 8.0 mg/kg increased the survival rate of mice by 28.6% to 71.2%; The yield of 6_keto_PGF1α increased by 56.3% to 21.0.8%, which reduced the output of TXB2 by 21.6 to 56.8%. The ratio of 6_keto_TGF1α to TXB2 was significantly increased in the control group, which was 1.15, and that of the drug group was 2.29, 3.13, 5.53, and 8.26. In vitro experiments demonstrated that XT 0.37 μmol/L, 0.18 85 μmol/L, 0.924 4 μmol/L, and 9.2 400 μmol/L significantly inhibited platelet aggregation and TXB 2 production. The inhibition rate of the former was 2 2 . From 7% to 55.3%, the latter ranged from 20.5 to 50.7%; however, the yield of 6_keto_PGF1α increased at the same time, with an increase rate of 27.3% to 47.0%. The ratio of 6_keto_PGF1α to TXB2 was 1:2.3 in the control group and 2.07, 24.4, 3.28, 3.85 in the drug group.