目的研究阴道毛滴虫(Trichomonas vaginalisvirus)对甲硝唑的耐药性,探索共生人型支原体对其耐药性的影响。方法由医院提供阴道毛滴虫样品,对其进行体外培养,达到纯培养后提取虫株核酸,进行扩增及测序;制备浓度梯度板,测定甲硝唑对阴道毛滴虫的最小致死浓度(minimum lethallon centration,MLC),并将人型支原体进行序列分析和清除后再测定DNA及MLC,并作前后比较。结果甲硝唑对34株阴道毛滴虫MLC范围为1~300μg/ml,其中敏感性较高的共有21株,9株敏感性略低,4株敏感性低;在人型支原体共生与甲硝唑MLC关系测定中,12株扩增出特异性产物(长度大小约为530bp),阳性率为35.3%。DNA检测阳性的12株阴道毛滴虫中,甲硝唑MLC在1~25μg/ml、50~70μg/ml和100~300μg/ml范围内的虫株共生率分别为14.3%、66.7%和75.0%。当MLC在300μg/ml时,有2个虫株呈现人型支原体共生现象,显示人型支原体共生状态与甲硝唑MLC之间存在一定的关联性;清除人型支原体后的甲硝唑MLC测定中,加入多西环素并对虫株进行传代培养。当传至3代后进行检测,12株阳性虫株中仅有7株未再检出人型支原体DNA,其中甲硝唑敏感株的4株MLC无变化,对甲硝唑耐药的3株MLC略有降低,但仍维持在较高水平。结论甲硝唑MLC不同的阴道毛滴虫虫株人型支原体的共生情况也不同。对甲硝唑耐药性越强的虫株人型支原体检出率越高,甲硝唑MLC为300μg/ml的虫株均查出人型支原体,表明阴道毛滴虫对甲硝唑的耐药性与人型支原体共生现象可能存在一定关系。 Objective To study the drug resistance of Trichomonas vaginalisvirus to metronidazole and explore its effect on the drug resistance of Mycoplasma hominis. Methods The samples of Trichomonas vaginalis were provided by the hospital and cultured in vitro. The pure nucleic acids of the strains were obtained and amplified and sequenced. The concentration gradient plate was prepared and the minimum lethal concentration of metronidazole to Trichomonas vaginalis minimum lethalon centration (MLC)). DNA and MLC were determined after sequence analysis and clearance of Mycoplasma hominis and compared before and after. Results The MLC of 34 strains of Trichomonas vaginalis ranged from 1 to 300 μg / ml, of which 21 were highly sensitive. The sensitivity of 9 strains was slightly lower and the sensitivity of 4 strains was lower. In the determination of nadiazole MLC, 12 strains amplified specific products (length of about 530bp), the positive rate was 35.3%. Among the 12 strains of Trichomonas vaginalis that tested for DNA, the symbiotic rates of metronidazole MLC in the range of 1 ~ 25μg / ml, 50 ~ 70μg / ml and 100 ~ 300μg / ml were 14.3%, 66.7% and 75.0% %. When MLC was 300μg / ml, there were 2 strains of Mycoplasma hominis showed symbiotic phenomenon, showing the relationship between the mycoplasma hominis and metronidazole MLC there is a certain correlation; Mycoplasma removal Mycoplasma MLC determination , Adding doxycycline and subculture of insect strains. When passed to the third generation and tested, only 7 out of 12 positive strains did not detect any Mycoplasma hominis DNA, of which 4 strains of metronidazole sensitive strains did not change and 3 strains were resistant to metronidazole MLC slightly lower, but still maintained at a high level. Conclusion Metronidazole MLC different Trichomonas vaginalis strains of Mycoplasma symbiosis situation is also different. The higher the resistance to metronidazole strains of Mycoplasma hominis the higher the detection rate, metronidazole MLC 300μg / ml of mycoplasma isolates were detected, indicating Trichomonas vaginalis resistance to metronidazole There may be some relationship between drug resistance and human mycoplasma symbiosis.