弓形虫的低温冷冻保存方法已为国内外学者采用。我们将弓形虫置于液氮保存,并对保存后虫体的超微结构进行观察。 材料和方法 NP株弓形虫系南京农业科学院畜牧兽医研究所自当地猪体内分离出的虫株,在本所用小鼠传代,每4d发病传代1次。收集感染NP株弓形虫小鼠的腹水和腹腔冲洗液,充分摇匀后用消毒安瓿分装,每 Toxoplasma cryopreservation methods have been used by scholars at home and abroad. We placed the Toxoplasma gondii in liquid nitrogen and observed the ultrastructure of the parasites after preservation. Materials and methods NP strain Toxoplasma gondii Department of Animal Husbandry and Veterinary Institute of Nanjing Academy of Agricultural Sciences from the local pig isolated strains of insects, the mice used in this passage, the incidence of disease every 4d passage 1. Collected Toxoplasma gondii NP mice infected with ascites and peritoneal washing fluid, shake well with sterile ampoules packaging, each