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目的利用比较基因组学研究河弧菌与霍乱弧菌阿拉伯糖发酵实验差异的分子遗传机制。方法比较河弧菌EF85003和霍乱弧菌N16961在以L-阿拉伯糖或葡萄糖为单一碳源的M9培养基中的生长曲线。对河弧菌EF85003进行基因注释,预测阿拉伯糖操纵子区域,利用比较基因组学两两比较河弧菌EF85003、弗尼斯菌NCTC11218和霍乱弧菌N16961序列。结果河弧菌EF85003可在以L-阿拉伯糖或葡萄糖为单一碳源的M9培养基中生长,而霍乱弧菌N16961仅在以葡萄糖为单一碳源的M9培养基中生长。比较基因组学研究发现河弧菌EF85003序列中VFL-003620基因至VFL-003628基因为阿拉伯糖操纵子区域,而霍乱弧菌N16961全基因组序列中不含完整的阿拉伯糖操纵子。结论河弧菌EF85003基因组上存在阿拉伯糖操纵子区域,霍乱弧菌N16961基因组序列中不含完整的阿拉伯糖操纵子,造成了河弧菌EF85003和霍乱弧菌N16961在阿拉伯糖发酵实验中的不同表型。
OBJECTIVE: To study the molecular genetic mechanism of the differences between V. cholerae and V. cholerae arabinose fermentation by comparative genomics. Methods The growth curves of V. cholerae EF85003 and V. cholerae N16961 were compared in M9 medium with L-arabinose or glucose as sole carbon source. The river Vibrio EF85003 was gene annotated to predict the Arabinose operon region, using comparative genomics to compare V. ampullae EF85003, N. melitensis NCTC11218 and V. cholerae N16961 sequences. As a result, vibrio cholerae EF85003 could grow in M9 medium with L-arabinose or glucose as a sole carbon source, whereas Vibrio cholerae N16961 was grown only in M9 medium with glucose as a sole carbon source. Comparative genomics revealed that the VFL-003620 to VFL-003628 genes in the V. cholerae EF85003 sequence were in the Arabian operon region, while the complete genome sequence of V. cholerae N16961 contained no complete arabinose operon. CONCLUSION: The presence of arabinose operon on the genome of V. tremori EF85003 does not contain intact arabinose operon in the genomic sequence of Vibrio cholerae N16961, resulting in the different table of V. cholerae EF85003 and V. cholerae N16961 in arabinose fermentation type.