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目的对一起感染性腹泻疫情的未知种型气单胞菌进行病原学鉴定。方法采集本起疫情腹泻患儿粪便标本用碱胨水增菌后接种于麦康凯平板,对可疑菌落进行系统生化鉴定;对鉴定为气单胞菌的菌株提取核酸,进行rpoB基因扩增及测序分析,所得序列在GenBank中进行同源性搜索并构建分子系统树,鉴定致感染性腹泻的气单胞菌基因种。结果 6份腹泻患儿粪便标本经培养后分离鉴定出4株气单胞菌,对rpoB基因测序后进行同源性搜索及系统发生分析,分离株气单胞菌为豚鼠气单胞菌。结论引起本次感染性腹泻疫情的病原菌为豚鼠气单胞菌。
Objective To identify aetiology of unknown species of Aeromonas aeruginosa together with an epidemic of infectious diarrhea. Methods Stool samples collected from infants with epidemic etiology were stained with Alkaline peptone and then inoculated on MacConkey plates to systematically identify the suspected colonies. Nucleic acids were extracted from the strains identified as Aeromonas, and rpoB gene was amplified and sequenced Analysis, the sequence obtained in GenBank homology search and the construction of molecular phylogenetic tree, identified to cause infectious diarrhea Aeromonas genotype. Results Stool samples of 6 children with diarrhea were isolated and identified 4 strains of Aeromonas. After homologous search and phylogenetic analysis of rpoB gene, Aeromonas isolates were Aeromonas caviae. Conclusion The pathogen causing the infectious diarrhea is Aeromonas caviae.