【摘 要】
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AIM:To investigate the in vitro effect of entecavir(ETV)on the function of dendritic cells(DCs)derived from chronic hepatitis B(CHB)patients.METHODS:Mononuclear
【机 构】
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Department of Gastroenterology,Department of Pathology and Molecular Medicine,Department of Biologic
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AIM:To investigate the in vitro effect of entecavir(ETV)on the function of dendritic cells(DCs)derived from chronic hepatitis B(CHB)patients.METHODS:Mononuclear cells were isolated from peripheral blood of patients with CHB.DCs were incubated with RPMI-1640 medium supplemented with fetal bovine serum,IL-4,granulocyte-macrophage colony-stimulating factor(GM-CSF).DCs were treated with or without ETV on the fourth day.Cell surface molecules,including CD1a,CD80,CD83 and HLA-DR,were assessed by flow cytometry.Concentrations of IL-6 and IL-12 in the supernatant were assayed by enzymelinked immunosorbent assay (ELISA).The ability of the generated DCs to stimulate lymphocyte proliferation was observed.RESULTS:Compared with CHB control group,the expression levels of CDla (29.07±3.20 vs 26.85±2.80),CD83 (25.66±3.19 vs 23.21±3.10),CD80(28.00±2.76vs 25.75±2.51) and HLA-DR (41.96±3.81 vs 32.20±3.04) in ETV-treated group were higher(P＜0.05).ETVtreated group secreted significantly more IL-12(157.60±26.85 pg/mL vs 132.60±22.00 pg/mL(P＜0.05) and had a lower level of IL-6 in the culture supematant (83.05±13.88 pg/mL vs 93.60±13.61 pg/mL,P＜0.05) than CHB control group.The ability of DCs to stimulate the proliferation of allogeneic lymphocytes was increased in ETV-treated group compared with CHB control group (1.53±0.09 vs 1.42±0.08,P＜0.05).CONCLUSION:Entecavir can enhance the biological activity of DCs derived from CHB patients.
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