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采用ISSR分子标记技术分析了湖南省5个地区6个居群白檀的遗传多样性水平和遗传结构。结果表明,9条ISSR引物对6个居群的149份白檀样品PCR扩增检测到122个扩增位点,其中多态性位点113个,多态性比率高达92.62%。Nei’s基因多样性指数(He)为0.3264,Shannon多样性指数(I)为0.4873,居群之间产生较大的遗传变异(Gst=0.5215,Nm=0.4588),AMOVA分子差异分析表明白檀居群间遗传分化程度高,51.07%的变异存在于居群间,48.93%的变异存在于居群内;UPGMA聚类分析将6个白檀居群分为3大类:大围山和龙山居群为一类,岳阳和道县居群组成另一类;衡山居群单独成一类,聚类原则跟地理位置不一致,与海拔高度有一定的相关性。
ISSR molecular markers were used to analyze the genetic diversity and genetic structure of the white sandalwood of six populations in five areas in Hunan Province. The results showed that 122 ISSR primer pairs were detected in 149 samples of white sandalwood from 6 populations by PCR amplification, including 113 polymorphic loci with a high polymorphism rate of 92.62%. Nei’s gene diversity index (He) was 0.3264, Shannon’s diversity index (I) was 0.4873, and there was a large genetic variation among populations (Gst = 0.5215, Nm = 0.4588) There was a high degree of genetic differentiation, with 51.07% variation among the populations and 48.93% variation within the population. UPGMA clustering analysis divided the six populations into three groups: Dawei Mountain and Longshan populations Yueyang and Daoxian populations form another group; Hengshan populations are grouped into one category, and the clustering principle is inconsistent with the geographical location and has some correlation with altitude.