论文部分内容阅读
目的 了解SLE患者外周血单一核细胞（PBMC）、外周血淋巴细胞（PBL）和单核细胞分泌白细胞介素-12(IL-12）的水平及 PBMC分泌 IL-12水平与 SLE活动性的关系。方法 体外培养 PBMC、PBL和单核细胞，予 LPS刺激后用夹心 ELISA法检测其上清中的 IL-12水平，用 SLEDAI估计 SLE的活动性。结果 SLE患者PBMC分泌 IL-12水平较正常对照低（P＜0．01），SLE患者和正常对照 PBL分泌的 IL-12差异无显著性（P＞0．05），而 SLE患者的单核细胞分泌的IL-12水平则显著低于正常对照（P＜0．01）。SLE患者PBMC分泌IL-12水平与SLEDAI呈负相关（P＜0．01）。结论 SLE患者PBMC分泌IL-12水平是降低的，在体外，SLE患者分泌IL-12水平降低是由单核细胞而不是B细胞的分泌能力降低引起的。且 IL-12水平可作为评价 SLE患者病情活动程度的参考指标。
Objective To investigate the relationship between the levels of IL-12 secreted by peripheral blood mononuclear cells (PBMC), peripheral blood lymphocytes (PBLs) and monocytes and the activity of SLE in patients with SLE . Methods PBMCs, PBLs and monocytes were cultured in vitro. The levels of IL-12 in supernatants were measured by sandwich ELISA. The activity of SLE was estimated by SLEDAI. Results The levels of IL-12 secreted by peripheral blood mononuclear cells in SLE patients were lower than those in normal controls (P <0.01). There was no significant difference in PBL secretion between SLE patients and normal controls (P> 0.05) The level of IL-12 secreted by cells was significantly lower than that of the normal control (P <0.01). The levels of IL-12 secreted by PBMC in SLE patients were negatively correlated with SLEDAI (P <0.01). Conclusions IL-12 secretion from PBMC is decreased in SLE patients. In vitro, the decrease of IL-12 secretion in SLE patients is caused by the decrease of secretory capacity of monocytes rather than B cells. And IL-12 levels can be used as a reference index to evaluate the degree of activity of SLE patients.