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运用RAPD分子标记技术分析了采自贵州各个地区的38个烟草黑胫病菌株的遗传多样性,探索了贵州省主要烟草种植区间的烟草黑胫病病菌的遗传分化关系。结果表明,受试38个菌株共产生65条谱带。其中多态性为59条,占90.7%。说明贵州省主要烟草种植区的烟草黑胫病病菌具有丰富的遗传多样性,不同地点烟草黑胫病病菌的遗传结构之间都有一定的差异。根据引物扩增的指纹图谱,运用UPGMA分析法,以遗传相似系数0.74,将供试38个菌株划分为9个基因型,发现除少数地区的菌株被划分为同一基因型外,其他不同地理区域的菌株基因型的划分与地理来源没有直接的相关性。
The genetic diversity of 38 tobacco blackleg strains collected from various regions of Guizhou Province was analyzed by RAPD molecular markers and the genetic differentiation of tobacco blackleg pathogen was explored in the main tobacco growing areas in Guizhou Province. The results showed that the test 38 strains produced a total of 65 bands. Among them, 59 were polymorphic, accounting for 90.7%. It shows that there are abundant genetic diversity in tobacco blackleg pathogen in the main tobacco growing areas of Guizhou Province. There is a certain difference between the genetic structure of tobacco blackleg pathogen in different locations. According to the fingerprinting of primer amplification, UPGMA analysis was used to divide the 38 isolates into 9 genotypes based on the genetic similarity coefficient of 0.74. It was found that in addition to the isolates belonging to the same genotype, the isolates from other geographical regions There is no direct correlation between the genotyping of strains and geographical origin.