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目的建立新的色谱技术,分离纯化紫草萘醌成分。方法采用中低压制备色谱和Flash柱进行分离纯化,利用理化性质和核磁共振谱、红外、质谱等技术鉴定结构,用高效液相色谱技术分析单体的纯度。结果分离鉴定了6个化合物。分别为去氧紫草素,紫草素,异丁酰紫草素,乙酰紫草素,β-乙酰氧基异戊酰紫草素,β,β-二甲基丙烯酰紫草素,纯度分别是99.62%,99.02%,99.5%,99.31%,99.3%,99.2%。结论首次建立中低压色谱技术,快速、准确分离紫草药材的单体成分。
Objective To establish a new chromatographic technique to separate and purify the components of radix naphthoquinone. Methods The compounds were separated and purified by medium and low pressure preparative chromatography (HPLC) and flash column. The structures were identified by physico-chemical properties, 1H-NMR, IR and MS-MS. The purity of the monomers was analyzed by high performance liquid chromatography. Results Six compounds were isolated and identified. Respectively were shikonin, shikonin, isobutyryl shikonin, acetylshikonin, β-acetoxyisovaleryl shikonin, β, β-dimethylacrylshikonin, purity Respectively 99.62%, 99.02%, 99.5%, 99.31%, 99.3%, 99.2%. Conclusion For the first time, low- and medium-pressure chromatographic techniques were established to rapidly and accurately separate the monomer components of Lithospermum.